MPEG-PGA，甲氧基聚乙二醇-聚谷氨酸的介绍（含参考文献）-CSDN博客

MPEG-PGA（甲氧基聚乙二醇-聚谷氨酸）

中文名称：甲氧基聚乙二醇-聚谷氨酸
英文名称：Methoxy Poly(ethylene glycol)-poly(L-glutamic acid)，简称 MPEG-PGA

材料结构与特点：
MPEG-PGA 是一种由甲氧基聚乙二醇（MPEG）和聚L-谷氨酸（PGA）构成的两嵌段共聚物，属于一种典型的亲水性-弱阴离子型高分子材料。MPEG 作为亲水头段，可改善材料的水溶性与生物相容性，防止非特异性蛋白吸附；而PGA链段则富含羧基，具备良好的功能修饰能力和药物负载性能。由于其整体结构可自组装成胶束或纳米粒子，在生理环境中稳定，已广泛用于药物控释领域。

制备方法：
MPEG-PGA 的合成通常采用开环聚合（ROP）策略。首先，选择具有羟基末端的 MPEG（MPEG-OH）作为引发剂，对γ-苄氧羰基-L-谷氨酸 N-羧基酐（BLG-NCA）进行开环聚合，形成 MPEG-b-PBLG。然后利用氢解或碱性水解去除 PBLG 上的保护基团（苄基），得到最终的 MPEG-PGA 嵌段共聚物。该合成方法具有可控聚合度、分子量分布窄、结构清晰的优点。

应用领域：

药物递送：MPEG-PGA 常用于负载药物（如紫杉醇、多柔比星等），在体内形成稳定胶束，实现控释和靶向释放。
蛋白质包裹与保护：利用PGA段的羧基可与蛋白形成非共价或共价相互作用，保护蛋白结构稳定性。
多功能纳米系统：通过接枝功能化分子（如靶向配体、荧光团等），可用于成像和治疗一体化系统。

发展前景：
MPEG-PGA 的良好生物降解性与低毒性使其在药物递送、纳米医学及组织工程中具有广泛前景。尤其在智能递送系统的开发中，PGA 段可设计为对 pH 或酶刺激敏感结构，实现肿瘤部位特异性释放。随着合成技术的提升及功能修饰策略的丰富，MPEG-PGA 有望发展成为多功能、多响应的医用高分子平台材料。

（5）参考文献

The synthesis and preparation of amphiphilie AA-conjugated polyethylene glyeol poly(lysine)polymerie micelle (mPEG-PLys-AA) utilized the method in our recent report[24] The gel pemmeationchromatography (GPC) analysis employed tetrahydrofuran as the eluent and polystyrene as thecalibration standard.(24] To obtin the Rsl3.lcaded micelles, 0.75 mg RsL3 together with 50 mgmPEG-PLys-AA were dissolved in CllCl, and mixed ria vortexing and sonication, Then the orginicsolvent was gently evaporated to achieve a thin film that was subsequently hydrated with 10 ml.deionized water, followed by sonieation fir 5 min, The excess RsL3 was removed by centrifugation andfiltration through a 0.45 pm membrane, finally, the micelle solution was lyophilized ind kept underargon at .20'c for further use. "The cryoprotectant ws not employed during lyophilization due to thestability of RsL3 and the difficulty of further purification post lyophiliztion. The RsL3 content in themicelles were analyzed by a high perfommance liguid chrmatogmphy (HPLC) (Waters e2695) coupledwith a photo diode aruy (PDA) detecor, In brief, the separation employed a Phenomenex Cl8 reversephase column (Gemini) which mnintained at 2$'C, The mobile phase was a mixture of seeonitrile and1%6 (vhv) acetie acid aqueous solution (60: 40, w'v) with a constint flow nate at l ml/min, The injectionvolume was 20 pl. and the detection wavelengths were 230 nm. The hydrodynamie size and morphologyof these polymerie mieelles was analyzed aecording to standard protocols by a Malvern Zetasizer Nano2S instrument and a JEM-100cX ll transmission cectron mieroscope, respectively.