I have a tiff image stack in uint16 datatype and I’d like to convert this to uint8 datatype. I’m not sure how to do this in Fiji.
I have loaded the stack in Fiji and I tried to change the datatype while exporting. But I couldn’t find any tab in Fiji export options for specifying the datatype.
Suggestions on how to do this in Fiji or alternatively in Python/MATLAB will be really helpful.
解决方案
The im2uint8() function can be used to convert the image from uint16 (unsigned integer 16) to uint8 (unsigned integer 8) in MATLAB.
For .tiff Files with a Single Image :
Image = imread("Test_Image.tiff");
Image = im2uint8(Image);
imshow(Image);
For .tiff Files with Multiple Images and Saving Transformed/Converted Images:
Reading the images in a loop using the imread() function with second argument being the Image_I