研究通过高通量蛋白质组学和转录组学方法揭示了EBV感染B细胞中HLA分子展示的抗原肽来源于功能相互关联的蛋白质，并且这些肽主要来自富含miRNA响应元件的转录本。不同个体的HLA展示的抗原肽谱虽有差异，但这些肽主要源自丰富的转录本，且呈现细胞类型特异性。此外，HLA分子展示的抗原肽产生与其转录本的丰度和DRiP率有关，而这个过程在很大程度上由miRNA调控。
摘要：
MHC I-associated () play an essential role in normal and diverse pathologic conditions. derive mainly from defective ribosomal products (DRiPs), a subset of nascent proteins that fail to achieve a proper conformation and the physical nature of which remains elusive. In the present study, we used high-throughput proteomic and transcriptomic methods to unravel the structure and biogenesis of presented by and molecules on EBV-infected B lymphocytes from 4 patients. We found that although HLA-different subjects present distinctive derived from different proteins, these originate from proteins that are functionally interconnected and implicated in similar biologic pathways. Secondly, the repertoire of B cells showed no bias toward conserved versus polymorphic genomic sequences, were derived preferentially from abundant transcripts, and conveyed to the a cell-type-specific signature. Finally, we discovered that derive preferentially from transcripts bearing miRNA response elements. Furthermore, whereas of HLA-disparate subjects are coded by different sets of transcripts, these transcripts are regulated by mostly similar miRNAs. Our data support an emerging model in which the generation of by a transcript depends on its abundance and DRiP rate, which is regulated to a large extent by miRNAs.
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