Ideal for Fluorescence Microscopy
High NA for Improved Throughput and Resolution
Excellent UV-NIR Performance (340-1000nm)
Long Working Distance
Featuring Zeiss’ unique ICS-Optics™ (Infinity Color-Corrected System) technique, these objectives achieve outstanding color correction throughout their entire wavelength range of 340-1000nm. The revolutionary design corrects for lateral chromatic aberration (LCA) in the tube lens, relieving the objective completely from LCA correction and reducing the required number of elements in the objective. Their high transmission of wavelengths from the near UV range (>40% starting at 340nm) and the use of glass types of low autofluorescence makes these objectives ideal for fluorescence microscopy. Each objective is designed for a 164.5mm tube lens focal length and features a 45mm parfocal length. Mounting thread: RMS (0.8" x 36TPI).
#58-395 and #58-452 accessory Tube Lenses can be used to focus light from the infinity-corrected objective onto the image plane. For optimal performance, the distance between the tube lens and the objective’s shoulder should be kept within 110±8mm and 130±8mm, respectively. This spacing allows accessories such as filters and beamsplitters to be inserted in the optical path to accommodate specific needs. #58-538 InfiniTube lens connects to these objectives via #53-786 RMS adapter.
Magnification
2.5X
5X
10X
Numerical Aperture (NA)
0.075
0.16
0.30
Working Distance (WD)
9.5mm
18.5mm
5.2mm
Focal Length
65.8mm
32.9mm
16.5mm
Cover Glass Thickness
0.17mm
0.17mm
0.17mm
Wavelength Range
340 - 1,000nm
340 - 1,000nm
340 - 1,000nm
Resolving Power
3.7µm
1.8µm
0.9µm
Depth of Field
62.4µm
15.6µm
3.9µm
Max Field Diameter
25.0mm
25.0mm
25.0mm
Field of View
Eyepiece, 25 Diameter Field
10.0mm
5.0mm
2.5mm
Eyepiece, 18 Diameter Field
7.2mm
3.6mm
1.8mm
2/3" Sensor Camera
3.52mm H x 2.64mm V
1.76mm H x 1.32mm V
0.88mm H x 0.66mm V
1/2" Sensor Camera
2.56mm H x 1.92mm V
1.28mm H x 0.96mm V
0.64mm H x 0.48mm V
Objective Thread
RMS (0.8" x 36 TPI)
RMS (0.8" x 36 TPI)
RMS (0.8" x 36 TPI)
Weight
156g
139g
92g
Magnification
20X LD
40X LD
63X LD
Numerical Aperture (NA)
0.40
0.60
0.75
Working Distance (WD)
8.4 - 7.4mm
3.3 - 2.5mm
2.2 - 1.2mm
Focal Length
8.2mm
4.1mm
2.6mm
Cover Glass Thickness
0 - 1.5mm
0 - 1.5mm
0 - 1.5mm
Wavelength Range
340 - 1,000nm
340 - 1,000nm
340 - 1,000nm
Resolving Power
0.7µm
0.5µm
0.4µm
Depth of Field
1.8µm
0.7µm
0.4µm
Max Field Diameter
25.0mm
25.0mm
25.0mm
Field of View
Eyepiece, 25 Diameter Field
1.25mm
0.625mm
0.40mm
Eyepiece, 18 Diameter Field
0.9mm
0.45mm
0.29mm
2/3" Sensor Camera
0.44mm H x 0.33mm V
0.22mm H x 0.17mm V
0.14mm H x 0.10mm V
1/2" Sensor Camera
0.32mm H x 0.24mm V
0.16mm H x 0.12mm V
0.10mm H x 0.08mm V
Objective Thread
RMS (0.8" x 36 TPI)
RMS (0.8" x 36 TPI)
RMS (0.8" x 36 TPI)
Weight
163g
233g
226g
Tube Lens Specifications
Focal Length
164.5mm
Design Wavelength
340-1000nm
Image Formation Magnification
1X
Image Field of View
25mm
Entrance Pupil Diameter
11mm
Objective Position Tolerance
±8mm
Diameter x Length
110mm Distance* (#58-395)
38.5mm x 6.5mm
130mm Distance* (#58-452)
40mm x 12.8mm
Mounting Thread
110mm Distance* (#58-395)
M36 x 0.5mm
130mm Distance* (#58-452)
M36 x 0.75mm
*Distance from lens shoulder to image = 162.07mm