signature=27fe40dfd620feec0260ffd4e7d59216,APS -68th Annual Meeting of the APS Division of Fluid Dyn...

68th Annual Meeting of the APS Division of Fluid Dynamics

Volume 60, Number 21

Sunday–Tuesday, November 22–24, 2015;

Boston, Massachusetts

Session R9: Microscale Flows: Microfluidic Devices II

12:50 PM–3:26 PM,

Tuesday, November 24, 2015

Room: 109

Chair: Matthew Hancock, Veryst Engineering

Abstract ID: BAPS.2015.DFD.R9.8

Abstract: R9.00008 : 3D flow focusing for microfluidic flow cytometry with ultrasonics

2:21 PM–2:34 PM

Authors:

Vaskar Gnyawali

(Department of Mechanical and Industrial Engineering, Ryerson University, Toronto, Canada)

Eric M. Strohm

(Department of Physics, Ryerson University, Toronto, Canada)

Yasaman Daghighi

(Department of Physics, Ryerson University, Toronto, Canada)

Mia Van de Vondervoort

(Department of Physics, Ryerson University, Toronto, Canada)

Michael C. Kolios

(Department of Physics, Ryerson University, Toronto, Canada)

Scott S.H. Tsai

(Department of Mechanical and Industrial Engineering, Ryerson University, Toronto, Canada)

We are developing a flow cytometer that detects unique acoustic signature

waves generated from single cells due to interactions between the cells and

ultrasound waves. The generated acoustic waves depend on the size and

biomechanical properties of the cells and are sufficient for identifying

cells in the medium. A microfluidic system capable of focusing cells through a

10 x 10 $\mu$m ultrasound beam cross section was developed to facilitate

acoustic measurements of single cells. The cells are streamlined in a

hydro-dynamically 3D focused flow in a 300 x 300 $\mu$m channel made

using PDMS. 3D focusing is realized by lateral sheath flows

and an inlet needle (inner diameter 100

$\mu$m). The accuracy of the 3D flow focusing is measured using a dye and

detecting its localization using confocal microscopy. Each flowing cell

would be probed by an ultrasound pulse, which has a center frequency of 375

MHz and bandwidth of 250 MHz. The same probe would also be used for

recording the scattered waves from the cells, which would be processed to

distinguish the physical and biomechanical characteristics of the cells,

eventually identifying them. This technique has potential applications in

detecting circulating tumor cells, blood cells and blood-related diseases.

To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2015.DFD.R9.8