linux ps prep,Collibri™ PCR-free PS DNA Library Prep Kit for Illumina Systems, with UD indexes (Set ...

描述

The Invitrogen Collibri PCR-Free PS DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems. The kit is suitable for physically sheared (PS) genomes of all sizes and variable quality, including FFPE samples.

The Collibri PCR-Free PS DNA Library Prep Kit:

• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library prep success

• Includes all necessary components in a single kit

• Offers improved coverage of challenging genomic regions compared to already available library prep technologies

• Supports PCR-free protocols starting with as little as 500 ng of DNA

• Provides up to 96 combinatorial dual or unique dual indexes

• Is applicable for automation of library preparation

The Collibri PCR-Free PS DNA Library Kit contains inert dyes that provide visual feedback during library preparation: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri PCR-Free PS DNA Library Prep Kit offers higher confidence SNP and INDEL calls resulting from stronger coverage of challenging genomic regions from inputs as low as 500 ng of physically sheared double-stranded DNA (see figure below). The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications

The Collibri PCR-Free PS DNA Library Prep Kit is recommended for:

• Whole genome sequencing

• Resequencing

• de novo assembly

For Research Use Only. Not for use in diagnostic procedures.