Column Title | Description |
|
Block | the block number of the feature. |
Column | the column number of the feature. |
Row | the row number of the feature. |
Name | the name of the feature derived from the Array List (up to 40 characters long, contained in quotation marks). |
ID | the unique identifier of the feature derived from the Array List (up to 40 characters long, contained in quotation marks). |
X | the X-coordinate in µm of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image. |
Y | the Y-coordinate in µm of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image. |
Dia. | the diameter in µm of the feature-indicator. |
F635 Median | median feature pixel intensity at wavelength #1 (635 nm). |
F635 Mean | mean feature pixel intensity at wavelength #1 (635 nm). |
F635 SD | the standard deviation of the feature pixel intensity at wavelength #1 (635 nm). |
B635 Median | the median feature background intensity at wavelength #1 (635 nm). |
B635 Mean | the mean feature background intensity at wavelength #1 (635 nm). |
B635 SD | the standard deviation of the feature background intensity at wavelength #1 (635 nm). |
% > B635 + 1 SD | the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #1 (635 nm). |
% > B635 + 2 SD | the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #1 (635 nm). |
F635 % Sat. | the percentage of feature pixels at wavelength #1 that are saturated. |
F532 Median | median feature pixel intensity at wavelength #2 (532 nm). |
F532 Mean | mean feature pixel intensity at wavelength #2 (532 nm). |
F532 SD | the standard deviation of the feature intensity at wavelength #2 (532 nm). |
B532 Median | the median feature background intensity at wavelength #2 (532 nm). |
B532 Mean | the mean feature background intensity at wavelength #2 (532 nm). |
B532 SD | the standard deviation of the feature background intensity at wavelength #2 (532 nm). |
% > B532 + 1 SD | the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #2 (532 nm). |
% > B532 + 2 SD | the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #2 (532 nm). |
F532 % Sat. | the percentage of feature pixels at wavelength #2 that are saturated. |
Ratio of Medians | the ratio of the median intensities of each feature for each wavelength, with the median background subtracted. |
Ratio of Means | the ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted. |
Median of Ratios | the median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted. |
Mean of Ratios | the geometric mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted. |
Ratios SD | the geometric standard deviation of the pixel intensity ratios. |
Rgn Ratio | the regression ratio of every pixel in a 2-feature-diameter circle around the center of the feature. |
Rgn R² | the coefficient of determination for the current regression value. |
F Pixels | the total number of feature pixels. |
B Pixels | the total number of background pixels. |
Sum of Medians | the sum of the median intensities for each wavelength, with the median background subtracted. |
Sum of Means | the sum of the arithmetic mean intensities for each wavelength, with the median background subtracted. |
Log Ratio | log (base 2) transform of the ratio of the medians. |
Flags | the type of flag associated with a feature. |
Normalize | the normalization status of the feature (included/not included). |
F1 Median - B1 | the median feature pixel intensity at wavelength #1 with the median background subtracted. |
F2 Median - B2 | the median feature pixel intensity at wavelength #2 with the median background subtracted. |
F1 Mean - B1 | the mean feature pixel intensity at wavelength #1 with the median background subtracted. |
F2 Mean - B2 | the mean feature pixel intensity at wavelength #2 with the median background subtracted. |
SNR 1 | the signal-to-noise ratio at wavelength #1, defined by (Mean Foreground 1- Mean Background 1) / (Standard deviation of Background 1) |
F1 Total Intensity | the sum of feature pixel intensities at wavelength #1 |
Index | the number of the feature as it occurs on the array. |
"User Defined" | user-defined feature data read from the GAL file (GenePix Pro 4.1). |