对山东三家AA级肉鸡养殖场的胚胎鸡、一日龄鸡和商品鸡进行CAV（鸡传染性贫血病毒）和ALV（鸡白血病病毒）检测，结果显示所有农场均有这两种病毒阳性感染，阳性率分别为24.22%和17.56%，共感染率为8.89%。病毒主要在脾脏和肾脏中检出。同时，发现肉鸡肝脏中分离出的病原体，且40日龄商品鸡的抗E.coli抗体滴度较低，表明存在多重感染现象。
摘要：
CAV (chicken infectious anemia virus) and ALV (avian leukosis virus) from embryonic chickens, one-day chickens and commercial chickens collected from 3 different AA broiler breeder farms of Shandong province were detected by PCR. DNA of the two viruses from different organs were extracted, amplified and then evaluated by PCR, and cloned plasmid was sequenced with the same way. The results showed that CAV and ALV were proved to be positive in the three farms investigated, and the positive ratio of CAV and ALV were 24.22%and 17.56% respectively. The co-infection ratio of ALV and CLV was 8.89%. Positive ratio differs in different organs, and the highest positive ratio of CAV and ALV appeared in spleen and kidney respectively. Meanwhile, we also isolated and identified pathogens from the liver of broilers.and at the same time, ND-HI test was used to detect valence of antibody to 40 d commercial chicken, it was showed that the infection ratio of E.coil was the highest and the valence of HI was lower.The above results demonstrated that CAV and ALV as well as some bacteria co-infected in broiler breeder and commercial chickens.
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