signature=d363d26bda212f777fef81d270ecd42b,The Correlation between Mice CD4~+CD25~+Foxp3~+ T Cells a...

The Correlation between Mice CD4~+CD25~+Foxp3~+ T Cells and GVHD

YANG Ming-zhen,WU De-pei,CHEN Guang-hua,CEN Jian-nong,YUAN Yan-hui,CHANG Hui-rong,ZHU Zi-ling,FENG Yu-feng(Department of Hematology,First Affiliated Hospital of Soochow University,Jiangsu institute of hematology,Suzhou 215006,China)

Objective To probe the correlation between mice CD4+CD25+Foxp3+ T cells and GVHD of mice allogeneic haemopoietic stem cell transplantation.Methods CB6F1 mice(H-2b/d,♀)were randomized to 3 groups between 8~10 weeks of age,weight was(20± 2)g,control group mice were not given X-ray and transplantation,autologous haemopoietic stem cell transplantation group mice received CB6F1 mice bone marrow cells(1× 107) and spleen cells(3×107)after X-ray irradiation,GVHD group mice were given C57BL/6(H-2b,♂)mice bone marrow cells(1 × 107) and spleen cells(3 × 107)after X-ray irradiation.At the time of GVHD happened,3 groups mice spleen cells were prepared,CD4+CD25+ T cells were detected with FCM(CD4-pcy5,CD25-FITC),the relative levels of foxp3 mRNA were determined by real-time quantitative RT-PCR in total splenocytes.Results The CD4+CD25+T cell of mice splenocytes in control group,auto-HSCT group and GVHD group was(8.47 ± 1.03)%,(15.40 ± 0.80) % and(24.03±0.85)%,respectively(P0.01),real-time quantitative RT-PCR showed that the levels of foxp3 mRNA of GVHD group mice splenocytes was lower than control group and auto-HSCT group(P0.001).Conclusion There was a negative correlation between CD4+CD25+Foxp3+T cells and GVHD,CD4+CD25+foxp3+T cells were lower when GVHD happened.

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