signature=22ccc9c9cb3389ea813a6db84c702f29,A Fast Identification Method of Notoginseng Radix Etrhizo...

摘要：

OBJECTIVE To establish a fast identification method of Panax notoginseng,molecular signature which can be used as fast authentication of P. notoginseng were screened according to comparison results of 248 Panax ITS2 sequences from Gen Bank and46 P. notoginseng ITS2 sequences from this study. METHODS ITS2 Sequences obtained in this study were assembled using Codoncode Aligner,and then were imported to MEGA 6. 0 together with Panax ITS2 sequences from Gen Bank. The comparison was conducted using MUSCLE method and then unique molecular signature of P. notoginseng was found in this comparison results. RESULTS The length of ITS2 sequences of P. notoginseng was 230 bp,and the aligned ITS2 sequences of Panax was 258 bp. Based on one SNP site of P. notoginseng,one unique molecular signature was developed,5'-AACCCATCAT TCCCTCGCGG GAGTCGATGC GGAGG-3'.If unknown samples contained the signature sequence after amplification,it would be identified to be Panax notoginseng and vice versa.CONCLUSION The molecular signature obtained from this study can be used for fast authentication of varying kinds of P. notoginsengcrude drugs and powders and authentication of mixed samples.

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