昆虫嗅觉神经的计算机三维重建 - 应用昆虫学报
# 668 # Chinese Bu etin of Entomo ogy 2008 45( 4)
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1, 2 1, 2**
付丙鲜 祝增荣
( 11 310029; 21 310029)
Computerized three dimensional reconstruction of the insect olfactory nerves. FU Bing- Xian 1, 2 , ZHU Zeng-
Rong1, 2** ( 11 Institute of InsectSciences, Zhejiang University, Hangzhou 310029 , China; 21 StateKeyLaboratory of
Rice Biology , Hangzhou 310029, China)
Abstract Based on aser scanning confoca microscope ( LSCM ) , the computerized three- dimensiona ( 3- D)
reconstructions of the insect o f actory nerves have been app ied in the insects of Orders Diptera, Lepidoptera,
Hymenoptera and B attaria. The nervous tissues abe ed by f uorescence can be optica y sectioned by LSCM , and a
seria of the optica sections can be used for 3- D reconstruction. T he map of antenna obe, as the primary o f actory
center of insects, is a prerequisite for comparison of the g omeru ar function among species and betw een ma e and
f ema e of cospecies. The morpho ogy and spatia re ationship of the principa neurons forming the o f actory pathw ays
are essentia for understanding the encoding procedure of odor information in the centra nervous system . The insect
o f actory nerves. 3- D reconstruction is of signif icance in exp oring the ro e of insect o faction in host se ection, food
f inding and partner connection.
Key words insect o factory nerve, antenna obe, aser scanning confoca microscope, optica section, three-
dimensiona reconstruction
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( Computerized