zblive.ml pc.html,2011 Stem cell treatment for patients with autoimmune disease by mesenchymal stem ...

Stem cell treatment for patients with

autoimmune disease by systemic infusion of culture-expanded autologous adipose tissue derived mesenchymal stem cells

Jeong Chan Ra 1*,Sung Keun Kang 1,Il Seob Shin 1,Hyeong Geun Park 1,Sang Aun Joo 1,Jeong Geun Kim 2,Byeong-Cheol Kang 3,Yong Soon Lee 4,Ken Nakama 5,Min Piao 6,Bertram Sohl 7and Andras Kurtz 8*

Introduction

In the 21st century,live expectancy has rapidly pro-gressed as has the number of previously uncommon dis-eases with no treatment.Stem cell based therapies are suggested to be able to repair and regenerate tissues in diseases associated with age,changed life style and envir-onmental exposure,such as autoimmune disease and

stroke.In particular,mesenchymal stem cells (MSCs)have been applied to treat these diseases [1-3].However,the lack of optimized culture protocols for achieving suf-ficient number of cells,safety issues concerning ex-vivo-expanded cells,the possible reduction in potency of stem cells derived from aged people and patients with autoim-mune disease has put into question clinical applications of autologous stem cells in these patients.

In order to apply human autologous adipose tissue derived MSC (hAdMSC)in the clinical setting,we developed a standardized protocol to isolate and cul-ture-expand AdMSC from minimal amounts of fat in vitro,achieving sufficient cell numbers for multiple

*Correspondence:jcra@rnl.co.kr;Andreas.Kurtz@charite.de 1

Stem Cell Research Center,RNL BIO,Seoul,153-768,Republic of Korea 8

Berlin-Brandenburg Center for Regenerative Therapies,Augustenburger Platz 1,13353Berlin,Germany and Seoul National University,College of Veterinary Medicine,Seoul,Korea

Full list of author information is available at the end of the article

Ra et al .Journal of Translational Medicine 2011,9:181

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