<!DOCTYPE html><html><head><metacharset="utf-8"><title></title><linkrel="stylesheet"type="text/css"href="CSS/subnav.css"/></head><body><!-- 导航栏部分 --><divclass="control"><divclass="left"><divclass="subNav"><divclass="part1 part"><ahref="#part1">part1</a></div><divclass="part2 part"><ahref="#part2">part2</a></div><divclass="part3 part"><ahref="#part3">part3</a></div><divclass="part4 part"><ahref="#part4">part4</a></div></div></div><!-- 正文部分 --><divclass="content"><aname="part1"></a><divclass="container1"><divclass="text1 text"><divclass="title1 title">title</div>
Doxycycline (doxycycline hyclate, doxycycline
hydrochloride or Dox), a synthetic tetracycline
(Tc) derivative, is the effector molecule for
Clontech's Tet-On® and Tet-Off® Inducible
Expression Systems. Dox can be used at
100-fold lower concentrations than Tc,
yielding inducing concentrations ranging
from 1ng/ml to 1 µg/ml. In the Tet-On System,
addition of Dox to culture medium induces a
conformational change in the transactivator
which allows it to bind to tet operator
sequences located in the inducible promoter.
This results in transcriptional activation of the
gene of interest. In the Tet-Off System, removal
of Dox from culture medium induces binding
of the transactivator, resulting in transcription
of the gene of interest.
</div></div><aname="part2"></a><divclass="container2"><divclass="text2 text"><divclass="title2 title">title</div>
Doxycycline (doxycycline hyclate, doxycycline
hydrochloride or Dox), a synthetic tetracycline
(Tc) derivative, is the effector molecule for
Clontech's Tet-On® and Tet-Off® Inducible
Expression Systems. Dox can be used at
100-fold lower concentrations than Tc,
yielding inducing concentrations ranging
from 1ng/ml to 1 µg/ml. In the Tet-On System,
addition of Dox to culture medium induces a
conformational change in the transactivator
which allows it to bind to tet operator
sequences located in the inducible promoter.
This results in transcriptional activation of the
gene of interest. In the Tet-Off System, removal
of Dox from culture medium induces binding
of the transactivator, resulting in transcription
of the gene of interest.
</div></div><aname="part3"></a><divclass="container3"><divclass="text3 text"><divclass="title3 title">title</div>
Doxycycline (doxycycline hyclate, doxycycline
hydrochloride or Dox), a synthetic tetracycline
(Tc) derivative, is the effector molecule for
Clontech's Tet-On® and Tet-Off® Inducible
Expression Systems. Dox can be used at
100-fold lower concentrations than Tc,
yielding inducing concentrations ranging
from 1ng/ml to 1 µg/ml. In the Tet-On System,
addition of Dox to culture medium induces a
conformational change in the transactivator
which allows it to bind to tet operator
sequences located in the inducible promoter.
This results in transcriptional activation of the
gene of interest. In the Tet-Off System, removal
of Dox from culture medium induces binding
of the transactivator, resulting in transcription
of the gene of interest.
</div></div><aname="part4"></a><divclass="container4"><divclass="text4 text"><divclass="title4 title">title</div>
Doxycycline (doxycycline hyclate, doxycycline
hydrochloride or Dox), a synthetic tetracycline
(Tc) derivative, is the effector molecule for
Clontech's Tet-On® and Tet-Off® Inducible
Expression Systems. Dox can be used at
100-fold lower concentrations than Tc,
yielding inducing concentrations ranging
from 1ng/ml to 1 µg/ml. In the Tet-On System,
addition of Dox to culture medium induces a
conformational change in the transactivator
which allows it to bind to tet operator
sequences located in the inducible promoter.
This results in transcriptional activation of the
gene of interest. In the Tet-Off System, removal
of Dox from culture medium induces binding
of the transactivator, resulting in transcription
of the gene of interest.
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