使用ImageJ进行两幅图像相减: Process-》image calculator-》substract,第一幅-第二幅 其他关于ImageJ使用: http://wenku.baidu.com/link?url=-vTViJTYfpzi8_-elFe9mJcTU_JA9fINfQm5cjtvTYXk36caoqcDy3s5ZgaV62wX83a4hd1p0Jji7QjodAR3HDa4TB1ioJKT6vHrDHvkgzW http://wenku.baidu.com/link?url=Z4vB1mzM_HMYMd2Ht1FrAvPRDhQEHPjH6XxweN6DCJYWG6OCvmSu_cRj1LRd5qHeYQfgBdV3ywT9pfq3kiUAfl-UhynEo-IbMRAYo3iwlXO