html发布机制tacat,microrna的作用与调控机制.ppt

microrna的作用与调控机制

SEED ????? ???? ??? ??? ????? ????? ??????? ???? ?????? ??????? ????? ???? ?? SEED ????? 13-130 ?????? ???????. ?????? ?? ??????? ?? Saetrum et. Al. * Neighboring seeds density CLDN12 AAGAAAACTTCTTGTAGCCTCACATTCCCCTTGTGCAAAGAGCTC TAGLN2 TAGATATATATTTTAGCAGTGACATTCCCAGAGAGCCCCAGAGCT ARCN1 GCCACAGTCTGTAATCCATTCACATTCCTCAGTTTCACCACCTCC TIP120A TTTCATTCCGTTTGGATGTCTACATTCCTTATCAAAGGATATAAA PGM2 TTATGTGTTTTACAAAGACCTACATTCCTCATTGTTTCATGTTTG -20 -10 0 10 20 * hsa-miR-302b hsa-miR-507 0.2 0.2 0 0.1 0.1 Seed density of window Seeds density = mean of targets density 0.12 Neighboring seeds density * Corrected α=0.00125 Overlapping seeds (miR binding sites) may interfere with miR function Neighboring seeds contribute to functionality Possibly favored periodical distances between seeds ? miR-mRNA binding pattern * We need a way to determine what is the preferable secondary structure of the miR-target complex RNAcofold from the Vienna package target 5' UUGGCUCACUUGCCUUAGTCATCGCTA 3' miRNA 3' ACCGUAAGUGGCGCACGGAAUU 5' target 5' UUGGUCAUUUUUUUUAUAUUGCCUUAU 3' miRNA 3' ACCGUAAGUGGCGCACGGAAUU 5' target 5' AUCAUAUUAAUUGUGCCUUAUAGTAAT 3' miRNA 3' ACCGUAAGUGGCGCACGGAAUU 5' (a) Using Vienna package RNAcofold we estimated the interaction between the miR and putative targets target 5' U G 3' UGGC UCACU UGCCUUA ACCG AGUGG ACGGAAU miRNA 3‘ UA CGC U 5‘ target 5' U U UUUUUAUAU U 3‘ UGG CAUUU UGCCUUA ACC GUAAG ACGGAAU miRNA 3‘ UGGCGC U 5‘ target 5' A AUAUUAAU U 3‘ UCA UGUGCCUUA AGU GCACGGAAU miRNA 3' ACCGUA GGC U 5' miR-mRNA binding pattern * Bound Not bound target 5' U G 3' UGGC UCACU UGCCUUA