64-bit versions of Windows use something called registry redirection and reflection keys. There is a compatibility layer called WoW64 which enables compatibility of 32-bit applications. Starting from Windows 7 and Windows Server 2008 R2 WoW64 registry keys are not longer reflected but shared. You can read about it here:
registry-reflection: msdn.microsoft.com/en-us/library/aa384235(v=vs.85).aspx
affected-keys: msdn.microsoft.com/en-us/library/aa384253(v=vs.85).aspx
wikipedia: en.wikipedia.org/wiki/WoW64
All you need to do is detect existence of those keys. You can use _winreg for that. Use try: and try opening key, example:
try:
aReg = _winreg.OpenKey(_winreg.HKEY_LOCAL_MACHINE,"SOFTWARE\\Wow6432Node\\Microsoft\\Windows\\CurrentVersion\\Run")