\documentclass[twocolumn]{article}%
\usepackage{color}
\setlength{\columnseprule}{1pt}
\newcommand{\latexcolumnseprulecolor}{\color{red}}
\usepackage{etoolbox}
\makeatletter
\patchcmd\@outputdblcol{% find
\normalcolor\vrule
}{% and replace by
\latexcolumnseprulecolor\vrule
}{% success
}{% failure
\@latex@warning{Patching \string\@outputdblcol\space failed}%
}
\makeatother
\usepackage{lipsum}
\begin{document}
\lipsum[1-5]
\end{document}
\documentclass{article}%
\usepackage{lipsum}
\usepackage{multicol}
\usepackage{color}
\setlength{\columnseprule}{1pt}
\renewcommand{\columnseprulecolor}{\color{red}}
\begin{document}
\begin{multicols}{2}
\lipsum[1-5]
\end{multicols}
\end{document}
本文选自how to change the color of the rule (vertical line) in twocolumn document?