IRDye 680RD NHS Ester/Maleimide/Carboxylate/Carboxylic acid，琥珀酰亚胺活化酯/马来酰亚胺/羧酸盐/羧酸

IRDye 680RD Infrared Dyes

IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals.

This infrared dye is ideal for labeling proteins, peptides, and antibodies. IRDye 680RD dye conjugates are optimized for protein detection applications, including quantitative Westerns and In-Cell Western™ assays. When used with IRDye 800CW dye-labeled agents, IRDye 680RD dye conjugates enable two-color imaging.

IRDye 680RD 红外染料

IRDye 680RD 红外染料是小动物成像应用的首选近红外荧光染料，波长为 700 nm，可在动物体内提供出色的清除曲线。

这种红外染料是标记蛋白质、肽和抗体的理想选择。 IRDye 680RD 染料偶联物针对蛋白质检测应用进行了优化，包括定量 Westerns 和 In-Cell Western™ 测定。 当与 IRDye 800CW 染料标记试剂一起使用时，IRDye 680RD 染料结合物可实现双色成像。

 

 

In-Cell Western Assay normalization with IRDye 680RD secondary antibody. A431 cells were treated with 2-fold serial dilutions of EGF (800-1.6 ng/mL). After fixation and permeabilization, cells were probed with rabbit anti-phospho EGFR and Mouse anti-ERK2 followed by detection with IRDye 800CW Goat anti-Rabbit IgG (P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (P/N 926-68070).

使用 IRDye 680RD 二抗进行 In-Cell Western Assay 标准化。 A431 细胞用 2 倍连续稀释的 EGF (800-1.6 ng/mL) 处理。 固定和透化后，用兔抗磷酸化 EGFR 和小鼠抗 ERK2 探测细胞，然后用 IRDye 800CW 山羊抗兔 IgG（P/N 926-32211）和 IRDye 680RD 山羊抗小鼠 IgG（P/N 926-68070）。

IRDye 680RD NHS Ester

IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein arrays, tissue section imaging, and molecular activity measurements.

IRDye 680RD NHS 酯

LI-COR Biosciences 的 IRDye 红外染料可供研究人员使用近红外 (NIR) 荧光开发应用。 这些独特的染料用于各种 NIR 成像应用，包括蛋白质印迹、基于板的测定、蛋白质阵列、组织切片成像和分子活性测量。

 

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

标准 NHS 酯化学用于生产用 LI-COR IRDye 红外染料标记的定制探针。 NHS 酯反应基团提供了标记伯胺和仲胺的功能，例如蛋白质中的赖氨酸残基。

IRDye 680RD Maleimide

IRDye 680RD Maleimide will label molecules with free sulfhydryl (-SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

IRDye 680RD 马来酰亚胺

IRDye 680RD 马来酰亚胺将标记具有游离巯基 (-SH) 基团的分子，例如蛋白质中的半胱氨酸残基。 缀合可以在生理pH下进行。

 

化学式：C45H48CIN5Na2O12S3

分子量：1028.51 g/mol

精确质量：1027.19

IRDye 680RD Carboxylate

IRDye 680RD 羧酸盐

 

 

使用 IRDye 680RD 偶联物的测定（例如小动物成像和细胞结合测定）可能需要“仅染料”对照来控制染料的潜在影响或保留。

IRDye 680RD 的羧酸盐（非反应性）形式是理想的对照。

化学式：C39H41CIN3Na3O11S3

分子量：928.37 g/mol

精确质量：927.13

IRDye 680RD Carboxylic acid

IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals.

This infrared dye is ideal for labeling proteins, peptides, and antibodies. IRDye 680RD dye conjugates are optimized for protein detection applications, including quantitative Westerns and In-Cell Western™ assays. When used with IRDye 800CW dye-labeled agents, IRDye 680RD dye conjugates enable two-color imaging.

IRDye 680RD 羧酸

IRDye 680RD 红外染料是小动物成像应用的首选近红外荧光染料，波长为 700 nm，可在动物体内提供出色的清除曲线。

这种红外染料是标记蛋白质、肽和抗体的理想选择。 IRDye 680RD 染料偶联物针对蛋白质检测应用进行了优化，包括定量 Westerns 和 In-Cell Western™ 测定。 当与 IRDye 800CW 染料标记试剂一起使用时，IRDye 680RD 染料结合物可实现双色成像。

 

 

In-Cell Western Assay normalization with IRDye 680RD secondary antibody. A431 cells were treated with 2-fold serial dilutions of EGF (800-1.6 ng/mL). After fixation and permeabilization, cells were probed with rabbit anti-phospho EGFR and Mouse anti-ERK2 followed by detection with IRDye 800CW Goat anti-Rabbit IgG (P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (P/N 926-68070).

使用 IRDye 680RD 二抗进行 In-Cell Western Assay 标准化。 A431 细胞用 2 倍连续稀释的 EGF (800-1.6 ng/mL) 处理。 固定和透化后，用兔抗磷酸化 EGFR 和小鼠抗 ERK2 探测细胞，然后用 IRDye 800CW 山羊抗兔 IgG（P/N 926-32211）和 IRDye 680RD 山羊抗小鼠 IgG（P/N 926-68070）。

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本文由QiyueLh整理，2022年6月2日