环境因素对亚硝酸盐氧化菌硝化活性的影响-CSDN博客

本文链接：https://blog.csdn.net/xiaohefeng123/article/details/122147809

1.Growth curve

Figure 3. Growth curve of the NOB culture
	NO2−-N		NO3−-N		nxrA
时间/h	mean	SD	mean	SD	mean	SD
0	104.7744	0.7002	20.7565	0.4076	26013.8604	457.3454
24	99.0563	0.4541	26.5786	0.1476	28398.7347	434.9381
48	83.8489	0.1429	42.8083	0.1205	65218.2563	2789.0500
60	73.3162	0.3430	53.0842	0.2007	266655.0680	11918.9382
72	60.4173	0.2645	65.1598	1.1043	557598.1493	34901.2365
80	46.1029	0.5799	80.0742	0.4112	981551.5816	17256.4963
88	26.1438	0.3510	98.1335	0.7404	1589550.0809	54979.7712
92	13.9397	0.2798	111.4618	0.4063	1803443.8539	45991.3210
96	0.1079	0.0044	125.6028	0.8391	1897598.5297	37940.1023

2.Temperature

Figure 4. Effect of temperature on the nitrification of nitrite-oxidizing bacteria.
	4℃		10℃		15℃		20℃		25℃		30℃		37℃		40℃
Time	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD
2 h	102.2295	2.8343	105.3581	2.4115	103.0893	4.9631	97.4245	1.8718	99.2175	1.2898	93.5095	2.7196	96.4729	1.1133	92.3587	0.4311
6 h	98.2961	1.3378	97.1348	0.7142	94.5960	2.3605	90.1790	3.8440	87.1763	4.3677	85.5314	1.5372	87.8801	1.0555	87.4803	1.9636
10 h	101.3251	0.7884	94.9342	3.8018	94.9421	1.9651	91.7938	2.1640	85.7948	4.1535	79.7814	2.9987	80.5586	3.7328	84.9756	1.0113
14 h	99.2804	0.6951	96.3904	0.5175	95.7586	0.8498	88.1816	1.5993	81.3214	2.1816	75.0642	1.7747	68.9892	4.9801	80.8037	2.0019
18 h	98.3263	2.0323	93.6366	1.3528	90.2551	4.6040	87.8998	0.7538	72.9016	6.6768	66.5172	2.2634	62.1723	6.4513	79.3056	2.6304
22 h	99.1979	1.2091	95.2697	1.5119	93.7074	3.6187	82.8720	1.5859	64.3808	3.1959	53.3173	1.1747	51.6908	5.1109	73.9895	1.3446
26 h	96.5280	2.7129	95.8045	0.9196	89.1829	3.4717	79.3122	1.8997	56.2100	2.8935	40.7387	1.6491	41.0926	6.0050	70.7442	2.5637
30 h	99.1612	0.4965	95.7875	0.6986	89.5342	2.3992	73.7942	2.6926	43.7952	3.4045	25.4247	0.4485	29.1064	5.6795	70.2357	1.0793
34 h	97.8347	1.0760	92.2407	0.2898	86.1657	1.6394	69.1832	2.0394	30.8457	1.4929	10.8263	0.9445	17.4649	5.3882	68.1962	2.5373
38 h	97.5726	1.1370	92.6038	1.3235	85.3046	2.3538	61.2050	2.2806	15.6889	4.0994	0.1849	0.2864	7.6334	4.8052	62.5747	2.0781
42 h	96.7312	0.7461	91.8371	0.5542	82.5744	2.4897	55.8883	2.6585	5.8141	1.0570			1.7922	2.1838	61.1487	1.6752
46 h	96.0037	0.9248	91.9642	0.7478	78.5139	3.0288	48.4324	2.4410							59.7738	2.2330

3.DO

Figure 5. Effect of dissolved oxygen on the nitrification activity of nitrite-oxidizing bacteria. A. Change of nitrite concentration in the medium; B. Change of nitrate concentration in the medium.

A. Change of nitrite concentration in the medium							B. Change of nitrate concentration in the medium
	4.5 ± 0.5 mg/L		6.8 ± 0.5 mg/L		1.0 ± 0.5 mg/L			4.5 ± 0.5 mg/L		6.8 ± 0.5 mg/L		1.0 ± 0.5 mg/L
Time	mean	SD	mean	SD	mean	SD	Time	mean	SD	mean	SD	mean	SD
0 h	21.3838	0.4646	21.2479	0.1032	21.2855	0.1809	0h	10.7864	0.2463	10.9385	0.0299	11.2763	0.2654
12 h	20.3661	0.3837	20.4668	0.0718	21.7410	0.6708	12h	11.0099	0.0490	11.0984	0.1892	10.8439	0.1118
24 h	19.7369	0.3841	19.8936	0.2645	23.8256	3.0657	24h	11.8470	0.2450	11.8548	0.1503	10.5262	0.4948
36 h	18.9643	0.5390	17.9546	0.1597	24.6422	1.9399	36h	12.1478	0.1182	12.4641	0.0532	9.7342	0.8565
48 h	16.4812	1.0955	14.7234	0.4420	24.5406	1.6061	48h	13.4351	0.2376	13.9489	0.3338	9.3507	0.8378
60 h	12.9738	1.4246	9.7489	0.1131	24.0674	1.7266	60h	15.1068	0.4161	16.7048	0.2797	9.0369	0.9456
72 h	9.7600	1.5329	5.8282	0.3115	23.3813	1.2142	72h	17.1232	0.7191	18.4775	0.4634	9.1329	1.4365
84 h	6.0252	1.6128	2.0499	0.2531	23.2496	1.4300	84h	19.2738	0.7861	20.2549	0.1281	8.5958	1.3178
92 h	3.6288	1.5070	0.4679	0.0948	23.7955	2.0160	92h	20.4694	0.4640	21.3471	0.3080	8.9985	2.4150
96 h	2.3914	1.3526	0.0681	0.0267	22.6919	2.3626	96h	21.3029	0.3951	21.8571	0.1304	9.5998	2.4651
100 h	1.5079	1.0930			23.3577	2.5654	100h	22.1197	0.3062			9.4086	2.3769

4.Salinity

Figure 6. Effect of salinity concentration on nitrification activity of nitrite-oxidizing bacteria. A. The salt used in the experiment was NaCl, the purity of which was more than 99.9%;B. The salt used in the experiment was commercial seawater crystal for aquaculture.

A. The salt used in the experiment was NaCl, the purity of which was more than 99.9%
	0.76‰		5.72‰		10.64‰		15.52‰		20.34‰		25.11‰		29.84‰
Time	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD
0 h	20.1114	0.2125	20.2094	0.7338	20.6179	0.9545	20.8215	0.6762	20.718	0.4935	20.638	0.4194	20.1067	0.4522
4 h	19.3208	0.385	19.5135	0.5737	20.6033	1.0362	20.3943	0.2811	21.3157	0.5342	21.469	0.6145	21.5811	0.3225
8 h	16.4596	0.4491	16.8339	0.672	16.8339	0.8789	18.6727	0.7119	19.442	0.3437	20.2147	0.5937	20.4906	0.2218
12 h	14.2917	0.2995	15.4825	0.8953	16.6326	0.7288	16.9414	0.4824	16.4986	0.2163	16.6779	0.4671	18.0749	0.4658
16 h	5.6445	0.2591	6.7146	0.4455	8.1459	0.5243	9.5385	0.4639	14.6729	0.2845	16.534	0.6678	17.4109	0.2929
20 h	2.4598	0.2231	3.6827	0.4455	6.4106	1.5799	9.8462	0.7826	13.6217	0.4235	15.4045	0.5254	16.9553	0.1883
24 h	0.0016	5.00E-04	0.6467	0.7288	5.9643	1.6121	8.0706	0.7231	12.097	0.239	15.0931	0.5533	16.4709	0.3221
28 h					1.8271	1.503	7.8376	0.2437	11.6866	0.5316	13.6914	0.643	16.3209	0.3934
32 h							4.6658	1.0472	9.8006	0.2468	13.6963	0.719	15.5009	0.4536
36 h							0.604	0.7122	8.752	0.6598	12.3689	0.6013	15.0915	0.356
40 h							0.0382	0.0502	6.6525	0.7884	10.7515	0.4284	14.7226	0.4304
44 h									3.8613	0.6547	10.8147	0.8387	13.9766	0.2786
48 h									1.0263	0.5383	9.0681	0.8982	11.6408	0.4544
52 h									0.0436	0.0335	3.3286	0.6015	10.518	0.4872
56 h											2.2331	0.1539	8.7601	0.6106
B. The salt used in the experiment was commercial seawater crystal for aquaculture.
	0.76‰		5.72‰		10.64‰		15.52‰		20.34‰		25.11‰		29.84‰
Time	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD
0 h	20.1114	0.2125	19.0537	0.4192	19.789	0.4242	20.1651	0.4453	20.259	0.4057	20.6711	0.2537	21.0289	0.4732
4 h	19.3208	0.385	18.921	0.5374	19.4368	0.1649	19.6228	0.5537	20.3602	0.5944	21.0994	0.2389	21.1073	0.4726
8 h	16.4596	0.4491	16.4556	0.3352	16.0124	0.6717	17.0586	0.3306	18.4879	0.6586	19.7029	0.3007	20.1682	0.6622
12 h	14.2917	0.2995	14.7118	0.4662	15.8848	0.3219	15.9576	0.6531	15.6517	0.4667	16.6596	0.3174	16.5783	0.3737
16 h	5.6445	0.2591	5.9662	0.1218	6.9309	0.2583	8.0961	0.189	11.3023	0.3656	14.9216	0.8085	16.2824	0.7181
20 h	2.4598	0.2231	2.9929	0.1264	4.6939	0.0914	7.1816	0.3739	10.0346	0.1931	13.0667	0.4107	15.0047	0.9609
24 h	0.0016	5.00E-04	0.0212	0.0045	2.7201	0.2477	6.7516	0.367	8.9834	0.343	11.826	0.2408	14.2204	0.738
28 h					0.0437	0.0133	3.1068	0.4009	8.6429	0.4745	11.0499	0.5373	13.4693	0.8937
32 h							0.1817	0.0357	5.1817	0.2665	9.9796	0.3854	12.2709	0.7633
36 h									0.9395	0.1635	7.8958	0.5762	11.161	0.896
40 h									0.0492	0.0106	5.826	0.6339	10.5726	0.5108
44 h											2.8754	0.6275	9.223	0.8062
48 h											0.3739	0.2448	6.6696	0.9045
52 h													2.084	0.2658
56 h													0.3426	0.0693

5.pH

Figure 7. Effect of different initial pH on nitrification activity of nitrite-oxidizing bacteria
		pH= 6 ± 0.1		pH= 7 ± 0.1		pH= 8 ± 0.1
	Time	mean	SD	mean	SD	mean	SD
	16 h	10.69355	0.0453	11.29614	0.03461	11.47832	0.14832
	24 h	10.83707	0.02202	11.18866	0.1642	11.13492	0.11418
	32 h	10.72632	0.12216	11.29646	0.14479	10.7001	0.2885
	40 h	10.40454	0.0913	11.01008	0.0538	10.44255	0.2493
	48 h	10.15551	0.1065	10.57919	0.1616	9.92385	0.3018
	56 h	9.61879	0.09947	10.25578	0.10557	9.70922	0.23014
	64 h	9.08862	0.12853	9.76034	0.13511	9.3963	0.36643
	72 h	8.61513	0.13279	9.29636	0.15398	8.82582	0.50422
	80 h	7.8238	0.2233	8.68853	0.08788	8.49553	0.47216
	88 h	6.55899	0.23826	7.36081	0.11756	7.71436	0.46683
	96 h	5.80011	0.25742	6.49379	0.17621	6.86209	0.52847
	104 h	4.66342	0.37837	4.97765	0.12127	5.35873	0.60796
	112 h	3.63518	0.47493	3.29178	0.14686	3.58898	0.62995
	120 h	2.76554	0.50298	1.95371	0.08331	2.20575	0.49594
	128 h	1.82126	0.4821	0.97941	0.05271	1.08276	0.37668
	132 h	1.42891	0.45834	0.63811	0.04679	0.62513	0.22602
	136 h	1.12109	0.45525	0.39511	0.03788	0.39714	0.18289
	140 h	0.84578	0.42526	0.19916	0.03252	0.18891	0.09473
	144 h	0.64112	0.37481	0.09163	0.02371	0.09318	0.04984

6.light

Figure 8. Effect of different wavelength light on nitrification activity of nitrite-oxidizing bacteria

A, Change of NO2--N concentration under 660 ± 10 nm light					B, Change of NO3--N concentration under 660 ± 10 nm light
	control		660 ± 10 nm			control		660 ± 10 nm
Time	mean	SD	mean	SD	Time	mean	SD	mean	SD
0 h	15.8750	0.1675	16.0801	0.1876	0 h	11.8356	0.1005	11.9695	0.1458
12 h	14.2059	0.1470	14.4883	0.4765	12 h	12.2245	0.2890	12.5440	0.3346
24 h	13.1285	0.5447	13.1285	1.2044	24 h	13.1799	0.1978	13.0715	0.3707
36 h	12.3047	0.9427	12.2909	1.8623	36 h	14.3616	0.2591	13.7217	0.5943
48 h	11.1887	1.4977	11.2188	2.7582	48 h	14.4588	0.8885	14.3129	1.4728
60 h	10.1021	1.8288	10.2761	3.4236	60 h	14.6811	1.1553	14.3273	1.9157
72 h	10.0087	2.1909	9.8413	3.9052	72 h	14.8564	1.2510	15.1504	1.8284
84 h	9.4726	2.4687	9.2420	4.3704	84 h	15.1377	1.2745	15.6102	2.0984
96 h	8.9946	2.5794	8.6849	4.8936	96 h	15.2118	1.5744	15.6118	2.6446
C, Change of NO2--N concentration under 550 ± 10 nm light					D, Change of NO3--N concentration under 550 ± 10 nm light
	Control		550 ± 10 nm			Control		550 ± 10 nm
Time	mean	SD	mean	SD	Time	mean	SD	mean	SD
0 h	15.0552	0.0561	14.9720	0.1405	0 h	7.7638	0.0775	7.7766	0.2917
12 h	14.0250	0.1389	14.9195	0.1553	12 h	7.9623	0.3186	7.8714	0.1015
24 h	12.8467	0.3012	14.6325	0.4187	24 h	7.9686	0.4333	7.5288	0.2025
36 h	11.3322	0.3199	14.4267	0.2995	36 h	8.2850	0.5094	7.5662	0.2735
48 h	10.2607	0.2237	14.3238	0.3784	48 h	8.6730	0.4372	7.9415	0.2513
60 h	9.0123	0.2680	13.9378	0.4925	60 h	9.2945	0.3674	8.2069	0.1054
72 h	8.5945	0.9715	13.3218	0.3588	72 h	10.3742	0.4217	8.3129	0.0738
84 h	7.0525	0.1804	12.8529	0.7016	84 h	10.9846	0.2619	8.5623	0.1011
96 h	6.1465	0.4042	11.9774	0.4158	96 h	11.7950	0.2967	8.9360	0.0325
E, Change of NO2--N concentration under 410 ± 10 nm light					F, Change of NO3--N concentration under 410 ± 10 nm light
	Control		410 ± 10 nm			Control		410 ± 10 nm
Time	mean	SD	mean	SD	Time	mean	SD	mean	SD
0 h	23.2692	0.1724	22.9842	0.1798	0 h	15.3671	0.1000	15.2667	0.1725
12 h	23.1133	0.2874	23.1362	0.2684	12 h	15.5568	0.0553	15.5162	0.1413
24 h	22.5031	0.3099	22.9147	0.3064	24 h	15.7337	0.1517	15.6333	0.1652
36 h	21.7770	0.6799	23.0589	0.1526	36 h	16.0500	0.0949	15.2835	0.3483
48 h	20.8969	0.4997	22.6250	0.6185	48 h	16.4723	0.3875	15.9122	0.5265
60 h	19.5967	1.0611	22.6906	0.4457	60 h	16.8445	0.4969	15.7624	0.7210
72 h	18.7670	0.8811	22.7974	0.4264	72 h	17.1122	0.5810	15.9249	0.7985
84 h	17.6385	0.6534	22.8420	0.3023	84 h	17.5018	1.3020	16.1560	1.1284
96 h	15.9897	1.0614	23.0176	0.1142	96 h	18.3568	1.6480	15.6915	1.9397