cd-hit报错解决

================================================================                                                                                                   
Program: CD-HIT, V4.8.1 (+OpenMP), Apr 07 2021, 10:57:21                                                                                                           
Command: cd-hit-est -i                                                                                                                                             
         /share/home/bmk530/bdz/seqkit/vircontig_merge.fa -o                                                                                                       
         /share/home/bmk530/bdz/cdhit/out/virunique.fa -c 0.95                                                                                                     
         -aS 0.85 -d 0 -T 20 -M 10000 -n 10                                                                                                                        

Started: Mon Feb 20 14:51:22 2023                                                                                                                                  
================================================================                                                                                                   
                            Output                                                                                                                                 
----------------------------------------------------------------                                                                                                   
total seq: 13313                                                                                                                                                   
longest and shortest : 258320 and 500                                                                                                                              
Total letters: 49590002                                                                                                                                            
Sequences have been sorted                                                                                                                                         

Approximated minimal memory consumption:                                                                                                                           
Sequence        : 51M                                                                                                                                              
Buffer          : 20 X 67M = 1344M                                                                                                                                 
Table           : 2 X 16M = 33M                                                                                                                                    
Miscellaneous   : 4M                                                                                                                                               
Total           : 1434M                                                                                                                                            

Table limit with the given memory limit:                                                                                                                           
Max number of representatives: 40000                                                                                                                               
Max number of word counting entries: 1070730103                                                                                                                    

# comparing sequences from          0  to       5000                                                                                                               
....---------- new table with     2919 representatives                                                                                                             
# comparing sequences from       5000  to       5377                                                                                                               
100.0%---------- new table with      179 representatives                                                                                                           
# comparing sequences from       5377  to       5737                                                                                                               
----------    125 remaining sequences to the next cycle                                                                                                            
---------- new table with      138 representatives                                                                                                                 
# comparing sequences from       5612  to       5962                                                                                                               
----------    181 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       5781  to       6123                                                                                                               
----------    169 remaining sequences to the next cycle                                                                                                            
---------- new table with      103 representatives                                                                                                                 
# comparing sequences from       5954  to       6288                                                                                                               
----------    137 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       6151  to       6476                                                                                                               
----------    117 remaining sequences to the next cycle                                                                                                            
---------- new table with      109 representatives                                                                                                                 
# comparing sequences from       6359  to       6675                                                                                                               
----------    124 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       6551  to       6858                                                                                                               
----------    110 remaining sequences to the next cycle                                                                                                            
---------- new table with      114 representatives                                                                                                                 
# comparing sequences from       6748  to       7046                                                                                                               
----------    141 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       6905  to       7196                                                                                                               
----------    124 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       7072  to       7355                                                                                                               
----------    109 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       7246  to       7521                                                                                                               
----------    107 remaining sequences to the next cycle                                                                                                            
---------- new table with      102 representatives                                                                                                                 
# comparing sequences from       7414  to       7682                                                                                                               
----------     84 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       7598  to       7857                                                                                                               
----------    101 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       7756  to       8008                                                                                                               
----------     93 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       7915  to       8160                                                                                                               
----------     75 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       8085  to       8322                                                                                                               
----------     89 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       8233  to       8463                                                                                                               
----------     75 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       8388  to       8611                                                                                                               
----------     68 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       8543  to       8759                                                                                                               
----------     23 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       8736  to       8944                                                                                                               
----------     57 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       8887  to       9088                                                                                                               
----------     52 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       9036  to       9230                                                                                                               
----------      5 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       9225  to       9410                                                                                                               
----------     26 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       9384  to       9562                                                                                                               
----------     13 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       9549  to       9720                                                                                                               
----------      9 remaining sequences to the next cycle                                                                                                            
---------- new table with      100 representatives                                                                                                                 
# comparing sequences from       9711  to       9874                                                                                                               
................---------- new table with       97 representatives                                                                                                 
# comparing sequences from       9874  to      10030                                                                                                               
........---------- new table with       95 representatives                                                                                                         
# comparing sequences from      10030  to      10179                                                                                                               
..........---------- new table with       89 representatives                                                                                                       
# comparing sequences from      10179  to      10321                                                                                                               
.............---------- new table with       75 representatives                                                                                                    
# comparing sequences from      10321  to      10457                                                                                                               
............---------- new table with       84 representatives                                                                                                     
# comparing sequences from      10457  to      10586                                                                                                               
.............---------- new table with       79 representatives                                                                                                    
# comparing sequences from      10586  to      10709                                                                                                               
..........---------- new table with       70 representatives                                                                                                       
# comparing sequences from      10709  to      10827                                                                                                               
.............---------- new table with       71 representatives                                                                                                    
# comparing sequences from      10827  to      10940                                                                                                               
.............---------- new table with       69 representatives                                                                                                    
# comparing sequences from      10940  to      11047                                                                                                               
............---------- new table with       56 representatives                                                                                                     
# comparing sequences from      11047  to      11150                                                                                                               
..........---------- new table with       59 representatives                                                                                                       
# comparing sequences from      11150  to      11248                                                                                                               
............---------- new table with       60 representatives                                                                                                     
# comparing sequences from      11248  to      11341                                                                                                               
............---------- new table with       57 representatives                                                                                                     
# comparing sequences from      11341  to      11430                                                                                                               
........---------- new table with       46 representatives                                                                                                         
# comparing sequences from      11430  to      11515                                                                                                               
...............---------- new table with       55 representatives                                                                                                  
# comparing sequences from      11515  to      11596                                                                                                               
.............---------- new table with       55 representatives                                                                                                    
# comparing sequences from      11596  to      11674                                                                                                               
............---------- new table with       52 representatives                                                                                                     
# comparing sequences from      11674  to      11748                                                                                                               
...........---------- new table with       39 representatives                                                                                                      
# comparing sequences from      11748  to      11819                                                                                                               
.............---------- new table with       45 representatives                                                                                                    
# comparing sequences from      11819  to      11886                                                                                                               
...........---------- new table with       42 representatives                                                                                                      
# comparing sequences from      11886  to      11950                                                                                                               
.............---------- new table with       43 representatives                                                                                                    
# comparing sequences from      11950  to      12011                                                                                                               
........---------- new table with       33 representatives                                                                                                         
# comparing sequences from      12011  to      12070                                                                                                               
............---------- new table with       33 representatives                                                                                                     
# comparing sequences from      12070  to      12126                                                                                                               
.........---------- new table with       33 representatives                                                                                                        
# comparing sequences from      12126  to      12179                                                                                                               
...............---------- new table with       38 representatives                                                                                                  
# comparing sequences from      12179  to      12230                                                                                                               
.............---------- new table with       37 representatives                                                                                                    
# comparing sequences from      12230  to      12279                                                                                                               
...........---------- new table with       42 representatives                                                                                                      
# comparing sequences from      12279  to      12326                                                                                                               
.............---------- new table with       39 representatives                                                                                                    
# comparing sequences from      12326  to      13313                                                                                                               
.....................                                                                                                                                              
Fatal Error:                                                                                                                                                       
file opening failed                                                                                                                                                
Program halted !!                                                                                                                                                  

---------- new table with      960 representatives 

报错内容显示文件打开失败

可以发现cd-hit-est是能够运行的,但是在输出的时候出现了问题

发现是因为输出路径中没有对应的文件夹,因此更改路径

cd-hit-est -i ~/bdz/seqkit/vircontig_merge.fa \
-o ~/bdz/cdhit/virunique \
-c 0.95 \
-aS 0.85 \
-d 0 \
-T 15 \
-M 10000 \
-n 10 \
; \
touch cdhit.sh.done

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