Python去除文档中重复的值，并按英文顺序输出

Python去除文档中重复的值，并按英文顺序输出

f = open('名单.txt', 'r')
line = f.readlines()
line = set(line)
line = sorted(line,key= lambda i:i[0])
for i in line:
    new = i.rstrip("\n")
    if i==line[len(line)-1]:      
        print(new)
    else:
        print(new,end=",")

名单.txt
啦啦啦
吼吼吼
嘤嘤嘤
锵锵锵
啊啊啊
不不不