【评测】NHEK细胞（正常成人表皮角质形成层细胞）

中文名称：正常成人表皮角质形成层细胞
英文名称：NHEK-Ad - Human Epidermal Keratinocytes, Adult, Single Donor
产品货号：00192627
1 x Cryopreserved ampule of NHEK-Ad from single donor containing ≥ 500,000 cells
Product Overview

Cryopreserved Normal Human Epidermal Keratinocytes are guaranteed through 18 population doublings for adult cells. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysi is provided for each cell lot purchased. 

Purchase our Adult Keratinocyte Cell Culture Kit (catalog no. 00192627B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of adult keratinocytes (catalog no. 00192627) and KGMTM-Gold BulletKitTM Growth Media (catalog no. 00192060)

中文名称：新生儿表皮角质形成层细胞-多供体混合
英文名称：NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
产品货号：00192906
Cryopreserved Human Neonatal Epidermal Keratinocytes, Pooled, ≥ 500,000 cells
Product Overview

Cryopreserved Normal Human Epidermal Keratinocytes are guaranteed through 18 population doublings for adult and neonatal cells.

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis |is provided for each cell lot purchased. 
 

References with Lonza’s Keratinocytes:

• Protective barrier: Keratinocytes were stimulated with histamine or histamine receptor ligands to show the formation of a defective skin barrier. Gschwandtner M, Mildner M,  Mlitz V,  Gruber F,  Eckhart L, Werfel T, Gutzmer R, Elias PM and Tschachler E.  Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model. European Journal of Allergy and Clinical immunology (2013); 68(1): 37–47. 

• Skin model: Neonatal keratinocytes were cultured in a microfluidic system to precisely examine the morphology, behavior and viability of individual cells. Adrian T. O’Neill, Nancy A. Monteiro-Riviere, and Glenn M. Walker. Characterization of microfluidic human epidermal keratinocyte culture. Cytotechnology (2008); 56(3): 197–207. 

• Cancer: Keratinocytes were used in this study to demonstrate that dysregulated activation of type I interferon innate immunity is implicated in the molecular processes triggered by anti-EGFR drugs and leading to persistent skin inflammation. Carrie HS, Hong Y, Yunbo L, Jennifer SM, Sridevi B, Gaylene S, Judith AC and Thomas RS.  EGF receptor signaling blocks aryl hydrocarbon receptor-mediated transcription and cell differentiation in human epidermal keratinocytes. Proceedings of National Academy of Sciences (2009); 106(11): 4266-71. 

• Human Papillomaviruses (HPV) mediated skin cancer response: An infection of the skin with human papillomaviruses (HPV) may result in benign tumors with limited growth, which tend to regress spontaneously and causes non-melanoma skin cancer development in epidermodysplasia verruciformis (EV) and immunosuppressed patients.  The present study utilized pooled keratinocytes cultured in KGMTM-Gold Medium from several healthy donors. The data showed that betaPV may increase the number of stem cell-like cells present during early carcinogenesis and thus enable the persistence and accumulation of DNA damage necessary to generate malignant stem cells. Martin H, Adrian B, Cinzia B, Marisa G, John D, Alan S, Herbert P, Ian M and Baki A. Expression of betapapillomavirus oncogenes increases the number of keratinocytes with stem cell-like properties. Journal of Virology (2013); 87(22): 12158-12165

高品质·始终如一
LONZA人原代细胞产品，包括人表皮角质形成层细胞、人原代单核细胞、肝原代细胞、神经元原代细胞等，美国原装进口，采用更严苛的质量管理标准，满足各类研究所需。

种类更丰富
LONZA提供多达200个不同类型组织细胞，包括从正常人、I型糖尿病人、II型糖尿病人、COPD病人、哮喘病人、肺囊性纤维化病人获取的同类组织原代细胞，解决人原代细胞获取困境。


品质更可靠
LONZA人表皮角质形成层细胞符合法律及伦理要求，对捐赠者个体信息、传染病及微生物检测、细胞活性和计数等细胞特征信息严格管理，生产标准化，品质稳定可靠，减少实验的不确定性，降低研究资金和时间成本。


运输安全
LONZA人表皮角质形成层细胞全程液氮运输，采用防倾倒液氮罐，由国外直接空运至中国，境内运输委托专业合作方，保证细胞质量始终如一。

人原代细胞培养基
LONZA为不同原代细胞提供经过优化的培养基、生长因子体系，可促进细胞增殖和分化，其低血清和无血清培养基，保持了正常细胞的功能，减少结果变异，与LONZA人表皮角质形成层细胞一起，形成1+1>2的原代细胞培养效果。

历史悠久
瑞士LONZA公司在全球具有40多个核心研发中心，全球员工10000余人，专注于制药与生物技术领域，从1984年开始生产人原代细胞产品，有30余年的生产经验，在欧美地区享有崇高的声誉，深受科研人员认可。

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