瘦素(Leptin)是由ob基因编码、在脂肪组织中生成的一种脂肪代谢调控产物,在代谢和调控体重等方面发挥重要作用。它通过下丘脑中的瘦素受体发出信号,降低食欲,增加能量消耗。在外周组织中,瘦素能拮抗胰岛素信号传导,增加脂肪酸氧化,减少胰岛β细胞的胰岛素分泌,并促进生育。它在脂肪细胞中的表达可以被其他脂肪因子如IL-6(刺激)和TNF-α(抑制)以旁分泌方式调节。瘦素缺乏的小鼠表现出严重的胰岛素抵抗、肥胖和生育能力下降,所有这些都可以通过外源性瘦素给药而逆转。还有研究表明,瘦素与心血管疾病有关,因为它介导了许多促炎和促动脉粥样硬化作用,如内皮素-1(ET-1)表达增加、活性氧(ROS)积累、单核细胞趋化蛋白-1(MCP-1)分泌以及交感血管张力和血压增加。
● 灵活:适用于正常和病理样本
● 高灵敏:可检测<26 pg/ml的小鼠瘦素水平
● 快速:仅需3小时就可获得39个样本的重复结果
● 方便:预包被板和即用型试剂为您节省实验时间
产品信息
产品货号 | ADI-900-019A / 欣博盛生物 |
产品名称 | Leptin (mouse), ELISA kit(小鼠瘦素ELISA试剂盒) |
规格 | 96 wells |
灵敏度 | 25.4 pg/ml |
检测范围 | 50-3,200 pg/ml |
检测时间 | 3 hours |
样本种属 | Mouse |
样本类型 | culture supernatants, plasma, and serum. Cited sample type includes tissue |
保存温度 | +4°C |
试剂盒组分 | Microtiter plate, Standard, Antibody, Conjugate, Assay buffer 28, TMB Substrate, Stop solution 2, Wash buffer concentrate |
部分产品引用文献
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2. eNAMPT actions through nucleus accumbens NAD+/SIRT1 link increased adiposity with sociability deficits programmed by peripuberty stress: L. Morató, et al.; Sci. Adv. 8, eabj9109 (2022)
3. Transcription factor CREB3 is a potent regulator of high-fat diet-induced obesity and energy metabolism: B.S. Smith, et al.; Int. J. Obes. 46, 1446 (2022)
4. The effects of mirabegron on obesity-induced inflammation and insulin resistance are associated with brown adipose tissue activation but not beiging in the subcutaneous white adipose tissue: C. Peres Valgas da Silva, et al.; Clin. Exp. Pharmacol. Physiol. 48, 1477 (2021)
5. Anti-Obesity Effects of Collagen Peptide Derived from Skate (Raja kenojei) Skin Through Regulation of Lipid Metabolism: M. Woo, et al.; Mar. Drugs 16, 306 (2018)
6. Anti-obesity effects of pectinase and cellulase enzyme‑treated Ecklonia cava extract in high‑fat diet‑fed C57BL/6N mice: I.H. Kim, et al.; Int. J. Mol. Med. 41, 924 (2018), Application(s): ELISA using mouse serum