Protein carbonyl ELISA kit羰基化蛋白ELISA试剂盒

蛋白质羰基化是蛋白质氧化损伤的一种，是氧化应激中一种不可逆的化学修饰。蛋白质羰基化会引起蛋白质结构的改变，使其失去原有的生物学功能，最终导致细胞和组织功能紊乱。蛋白质羰基化不仅是氧化损伤的一项重要指标，也参与多种疾病的生理和病理过程，比如衰老，凋亡，神经退行性疾病等。蛋白质样品中蛋白质羰基化的量可以用二硝基苯肼(DNP)衍生化并用比色法或免疫法检测结合的DNP来测定。ELISA方法能够用微克蛋白质定量测检测羰基化合物。

Enzo Life Sciences / 欣博盛生物提供的Protein carbonyl ELISA kit是一种于抗体的检测试剂盒。与比色分析相比，它具有更高的灵敏度、更低的背景、可检测更多的样本。

产品特点

◆ 高灵敏度ELISA，可检测低水平的羰基化蛋白

◆ 已在多种类型样本中验证

◆ 超越半定量Western Blot分析的全定量检测

产品信息

产品货号	ALX-850-312-KI01
产品名称	Protein carbonyl ELISA kit / 欣博盛生物
规格	96 wells
检测时间	4.5 hours
适用样本类型	血浆，其他体液，细胞和组织提取物
适用种属	不限种属
检测波长	450 nm

标准曲线示例

部分产品文献引用

1. Cardiovascular and Blood Oxidative Stress Responses to Exercise and Acute Woodsmoke Exposure in Recreationally Active Individuals: C.M. Williamson-Reisdorph, et al.; Wilderness Environ. Med. 33, 17-31 (2022)

2. Preliminary experience on the use of sucrosomial iron in hemodialysis: focus on safety, hemoglobin maintenance and oxidative stress: F. Reggiani, et al.; Int. Urol. Nephrol. 10, 11255 (2021)

3. A longitudinal study of neurotrophic, oxidative, and inflammatory markers in first-onset depression in midlife women: M.A. Pasquali, et al.; Eur. Arch. Psychiatry Clin. Neurosci. 268, 771 (2018), Application(s): ELISA using human serum

4. Antibiotic bedaquiline effectively targets growth, survival and tumor angiogenesis of lung cancer through suppressing energy metabolism: X. Wu, et al.; Biochem. Biophys. Res. Commun. 495, 267 (2018), Application(s): Human lung cancer cells

5. Antibiotic drug piperacillin induces neuron cell death through mitochondrial dysfunction and oxidative damage: S. Jiang, et al.; Can. J. Physiol. Pharmacol. 96, 562 (2018), Application(s): SH-SY5Y cell lysates

6. Plasma protein carbonylation in haemodialysed patients: focus on diabetes and gender: G. Colombo, et al.; Oxid. Med. Cell. Longev. 2018, 4149681 (2018), Application(s): ELISA using human plasma

7. The biological age linked to oxidative stress modifies breast cancer aggressiveness: M.D.M. Saez-Freire, et al.; Free Radic. Biol. Med. 120, 133 (2018), Application(s): ELISA using liver homogenates

8. Tigecycline as a dual inhibitor of retinoblastoma and angiogenesis via inducing mitochondrial dysfunctions and oxidative damage: Y. Xiong, et al.; Sci. Rep. 8, 11747 (2018)

9. Antibiotic tigecycline enhances cisplatin activity against human hepatocellular carcinoma through inducing mitochondrial dysfunction and oxidative damage: J. Tan, et al.; Biochem. Biophys. Res. Commun. 483, 17 (2017), Application(s): HCC cell lysates

10. Induction of Mitochondrial Dysfunction and Oxidative Damage by Antibiotic Drug Doxycycline Enhances the Responsiveness of Glioblastoma to Chemotherapy: Q. Tan, et al.; Med. Sci. Monit. 23, 4117 (2017), Application(s): Human glioblastoma cell lines A172 and U87 lysates

11. Targeting mitochondrial respiration selectively sensitizes pediatric acute lymphoblastic leukemia cell lines and patient samples to standard chemotherapy: X. Fu, et al.; Am. J. Cancer Res. 7, 2395 (2017)

12. Impact of chromium dinicocysteinate supplementation on inflammation, oxidative stress, and insulin resistance in type 2 diabetic subjects: an exploratory analysis of a randomized, double-blind, placebo-controlled study: Z.M. Saiyed, et al.; Food Nutr. Res. 60, 31762 (2016), Application(s): Measurement of biomarkers in plasma