BioXcell InVivoPlus anti-mouse Ly6G及部分参考文献

BioXcell InVivoPlus anti-mouse Ly6G 1A8单克隆抗体与小鼠Ly6G反应。Ly6G分子量为21-25kDa，是GPI锚定的细胞表面蛋白Ly-6超家族的成员，在细胞信号传导和细胞粘附中发挥作用。Ly6G在发育过程中由骨髓谱系中的细胞（包括单核细胞、巨噬细胞、粒细胞和嗜中性粒细胞）差异表达。单核细胞通常在发育过程中瞬时表达Ly6G，而成熟的粒细胞和外周嗜中性粒细胞保持表达，使Ly6G成为这些群体的良好细胞表面标志物。与BioXcell RB6-8C5抗体不同，1A8抗体与小鼠Ly6G特异性反应，而与Ly6C没有交叉反应性的报道。

 产品详情

产品名称	InVivoPlus anti-mouse Ly6G/欣博盛生物
产品货号	BP0075-1
产品规格	5/25/50/100mg
反应种属	Mouse
克隆号	1A8
同种型	Rat IgG2a, κ
免疫原	EL4J cells transfected with Ly6G
实验应用	in vivo neutrophil depletion in vivo MDSC depletion Immunofluorescence Immunohistochemistry (paraffin) Immunohistochemistry (frozen) Flow cytometry
产品形式	PBS, pH 7.0,Contains no stabilizers or preservatives
纯度	>95%, Determined by SDS-PAGE
聚合	<5%, Determined by SEC
无菌处理	0.2 µm filtration
纯化方式	Protein G
RRID	AB_1107721
分子量	150 kDa
小鼠病原检测	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
保存条件	抗体原液保存在4°C，不能冷冻保存。
推荐同型对照	InVivoPlus rat IgG2a isotype control, anti-trinitrophenol（货号BP0089）
推荐抗体稀释液	InVivoPure pH 7.0 Dilution Buffer（货号IP0070）

 

为什么选择InVivoPlus抗体？

InVivoPlus级别的产品内毒素含量更低，经过多种实验验证，更适合用于体内实验研究

 

该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：

应用	文章
体内中性粒细胞耗竭 (in vivo neutrophil depletion)	1. Davis, R. W. t., et al. (2018). 'Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic Efficacy' Photochem Photobiol . 2. Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses' Nat Med. doi : 10.1038/nm.4200. 3. Conde, P., et al. (2015). 'DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance' Immunity 42(6): 1143-1158. 4. Griseri, T., et al. (2015). 'Granulocyte Macrophage Colony-Stimulating Factor-Activated Eosinophils Promote Interleukin-23 Driven Chronic Colitis' Immunity 43(1): 187-199. 5. Yamada, D. H., et al. (2015). 'Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection' Immunity 42(2): 379-390. 6. Ellis, G. T., et al. (2015). 'TRAIL+ monocytes and monocyte-related cells cause lung damage and thereby increase susceptibility to influenza-Streptococcus pneumoniae coinfection' EMBO Rep 16(9): 1203-1218. 7. Deshmukh, H. S., et al. (2014). 'The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice' Nat Med 20(5): 524-530.
体内中性粒细胞耗竭、流式细胞术、免疫组化石蜡切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin))	Coffelt, S. B., et al. (2015). 'IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis' Nature 522(7556): 345-348.
体内中性粒细胞耗竭、流式细胞术、免疫组化石蜡切片、免疫组化冰冻切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin), Immunohistochemistry (frozen))	Finisguerra, V., et al. (2015). 'MET is required for the recruitment of anti-tumoural neutrophils' Nature 522(7556): 349-353.
体内中性粒细胞耗竭、流式细胞术(in vivo neutrophil depletion, Flow Cytometry)	1.Moser, E. K., et al. (2014). 'Late engagement of CD86 after influenza virus clearance promotes recovery in a FoxP3+ regulatory T cell dependent manner' PLoS Pathog 10(8): e1004315. 2. Chen, K. W., et al. (2014). 'The neutrophil NLRC4 inflammasome selectively promotes IL-1beta maturation without pyroptosis during acute Salmonella challenge' Cell Rep 8(2): 570-582. 3. Garraud, K., et al. (2012). 'Differential role of the interleukin-17 axis and neutrophils in resolution of inhalational anthrax' Infect Immun 80(1): 131-142. 4. Lee, W. B., et al. (2012). 'Neutrophils Promote Mycobacterial Trehalose Dimycolate-Induced Lung Inflammation via the Mincle Pathway' PLoS Pathog 8(4): e1002614.
体内骨髓来源抑制细胞耗竭(in vivo MDSC depletion)	Deng, L., et al. (2014). 'Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice' J Clin Invest 124(2): 687-695.
体内中性粒细胞耗竭、免疫荧光(in vivo neutrophil depletion,Immunofluorescence)	Edelson, B. T., et al. (2011). 'CD8alpha(+) dendritic cells are an obligate cellular entry point for productive infection by Listeria monocytogenes' Immunity 35(2): 236-248.