FITC-L-Glutamine,FITC-L-谷氨酰胺,用于观察谷氨酰胺在细胞或组织中的分布

文章关键词:FITC-L-谷氨酰胺,FITC-L-Glutamine,Fluorescent -L-Glutamine

一、基本信息

【性质简介】:FITC-L-Glutamine, the combination product of fluorescent dye FITC and glutamine, is a biochemical tool with special application value. FITC, also known as fluorescein isothiocyanate, is a widely used fluorescent dye. Its unique chemical structure endows it with the characteristic of emitting bright green fluorescence under specific light source excitation. This characteristic makes FITC an indispensable tool in the field of biomedical research. Glutamine, as an important amino acid, plays a crucial role in cellular metabolism and physiological functions. In order to gain a deeper understanding of the distribution and uptake of glutamine in cells or tissues, researchers cleverly utilized the fluorescence characteristics of FITC and coupled it with glutamine. Through this innovative combination, FITC-L-Glutamine not only retains the biological activity of glutamine, but also endows it with fluorescent labels that are easy to observe and track. In the experiment, when FITC-L-Glutamine is introduced into cell or tissue samples, researchers can observe its distribution through a specific fluorescence microscope. Under appropriate light source excitation, FITC-L-Glutamine emits bright green fluorescence, clearly displaying the position and quantity of glutamine in the sample.

【中文名】:FITC-L-谷氨酰胺

【英文名】:FITC-L-Glutamine,Fluorescent -L-Glutamine

【包装图】:

 

二、详情介绍

【外观】:固体/粉末

【溶解性】:溶于大部分有机溶剂

【规格】:10mg、25mg、50mg

【纯度】:95%+

【储存条件】:本品应密封避光,储存于阴凉、干燥、通风处,避免反复冻融,储液应该立即使用

【注意事项】:试剂仅用于科学研究或者工业应用等非医疗目的,非药用,非食用!

三、相关试剂

Cyanine5 azide,Cy5-N3

Cyanine5.5 NHS ester,Cy5.5 NHS,Cyanine5.5 SE,Cy5.5 SE

Cyanine5 maleimide,Cy5 Mal

Cyanine5 amine,Cy5-NH2

Cyanine5.5 azide,Cy5.5-N3

Cyanine5.5 amine,Cy5.5 NH2

Cyanine5 NHS ester

Cyanine5 hydrazide,Cy5 HZ

Cyanine5 DBCO,Cy5 DBCO

Cyanine5.5 DBCO,Cy5.5 DBCO

Cyanine5.5 maleimide,Cy5.5 Mal

Cyanine5 alkyne,Cy5 Alkyne

Cyanine5 tetrazine,Cy5 tetrazine

本文整理分享来自于小编zmy

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OpenCV可以通过色彩空间转换函数和图像分割函数来实现光谱拆分应用示例-FITC检测。 首先,将彩色图像转换为HSV色彩空间,HSV色彩空间的H通道可以表示颜色的色相,S通道可以表示颜色的饱和度,V通道可以表示颜色的亮度。然后,根据需要对图像进行阈值分割,得到二值图像。最后,根据二值图像提取感兴趣区域并进行处理。 下面是一个简单的示例代码,用于检测FITC标记的细胞: ```python import cv2 # 读取彩色图像 image = cv2.imread('cell.jpg') # 将彩色图像转换为HSV色彩空间 hsv = cv2.cvtColor(image, cv2.COLOR_BGR2HSV) # 设置阈值,提取FITC标记的细胞 low_green = (50, 50, 50) high_green = (70, 255, 255) mask = cv2.inRange(hsv, low_green, high_green) # 对二值图像进行形态学操作,去除噪点 kernel = cv2.getStructuringElement(cv2.MORPH_RECT, (5, 5)) mask = cv2.morphologyEx(mask, cv2.MORPH_OPEN, kernel) # 提取感兴趣区域 contours, hierarchy = cv2.findContours(mask, cv2.RETR_EXTERNAL, cv2.CHAIN_APPROX_SIMPLE) # 绘制感兴趣区域 for contour in contours: cv2.drawContours(image, [contour], 0, (0, 255, 0), 2) # 显示结果 cv2.imshow('FITC Detection', image) cv2.waitKey(0) cv2.destroyAllWindows() ``` 在上述代码,`cv2.cvtColor`函数用于将彩色图像转换为HSV色彩空间,`cv2.inRange`函数用于根据阈值提取FITC标记的细胞,`cv2.morphologyEx`函数用于对二值图像进行形态学操作,去除噪点,`cv2.findContours`函数用于提取感兴趣区域,并使用`cv2.drawContours`函数绘制感兴趣区域。最后使用`cv2.imshow`函数显示结果。 注意,在使用`cv2.findContours`函数时,需要根据OpenCV的版本进行调整。在OpenCV 3.x版本,`cv2.findContours`函数返回两个值,而在OpenCV 4.x版本,`cv2.findContours`函数只返回一个值。
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