1. 输出原序列的反向互补序列
in1 = open("brca1.fasta", "r") out1 = open("re_brca1.fasta", "w") raw = in1.readlines() # 序列信息暂时不想改 out1.write(raw[0]) s = raw[1].split("\n")[0] # 原序列中存在小写,先统一改为大写 s = s.upper() # 不指定替换次数,所以全部替换 s.replace('G', 'C') s.replace('C', 'G') s.replace('A', 'T') s.replace('T', 'A') # 逆序输出,再加个换行符:) out1.write(s[::-1]+"\n") out1.close() in1.close()
2. 读取大量数据:试了2millions行的文件,ok
with open('probes.blast.txt') as file: for line in file: