生物素-四聚乙二醇-二苯基环辛炔(DBCO-PEG4-biotin)是一种化合物,由以下三部分组成:
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生物素(Biotin):生物素是一种维生素,常用作生物化学分析中的探针,因其与亲和素和链霉亲和素(streptavidin)具有很高的亲和力而闻名。
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四聚乙二醇(PEG4,Tetraethylene glycol):四聚乙二醇是一种聚乙二醇,其中包含四个乙二醇单元。它通常用作连接物,以增加生物分子的溶解性和柔韧性。
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二苯基环辛炔(DBCO,Dibenzocyclooctyne):二苯基环辛炔是一种反应基团,可与偶氮基在无铜点击化学反应中形成稳定的共价键。这种反应被称为张力促进偶氮基-炔基环加成反应(SPAAC),由于其在生理条件下的生物正交性和高效性而被广泛应用于生物偶联。
将这三种组分结合起来,DBCO-PEG4-biotin成为一种用于将生物素标记到蛋白质、核酸和脂质等生物分子上的多功能工具,用于亲和纯化、荧光成像和药物输送等各种应用中。
DBCO-PEG4-biotin, also known as biotin-PEG4-DBCO, is a compound used in bioconjugation and chemical biology. It consists of three components:
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Biotin: Biotin is a vitamin that is commonly used as a probe in various biochemical assays due to its high affinity for avidin and streptavidin.
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PEG4 (Tetraethylene glycol): PEG4 is a polyethylene glycol with four repeating ethylene glycol units. It is often used as a linker to increase the solubility and flexibility of biomolecules.
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DBCO (Dibenzocyclooctyne): DBCO is a reactive group that forms a stable covalent bond with azide groups in a copper-free click chemistry reaction known as strain-promoted azide-alkyne cycloaddition (SPAAC). This reaction is widely used for bioconjugation due to its bioorthogonality and high efficiency under physiological conditions.
When combined, DBCO-PEG4-biotin forms a versatile tool for labeling biomolecules such as proteins, nucleic acids, and lipids with biotin for various applications such as affinity purification, fluorescence imaging, and drug delivery.