【齐岳分享】Maleimide-PEG2-Biotin 马来酰亚胺-二聚乙二醇-生物素

马来酰亚胺-二聚乙二醇-生物素（Maleimide-PEG-Biotin）是一种常用于生物偶联和化学生物学研究中的化合物。它由三个组分构成：

1. 马来酰亚胺（Maleimide）：马来酰亚胺是一种反应基团，通常用于与蛋白质中的巯基（-SH）发生特异性共价键结合。这种反应形成的硫醚键在温和条件下稳定，适用于生物分子的特异性标记和偶联。

2. 二聚乙二醇（PEG，Polyethylene Glycol）：二聚乙二醇是一种聚合物，通常用作生物分子之间的连接物。它可以增加生物分子的溶解度和稳定性，并提高它们在生物体内的循环时间。

3. 生物素（Biotin）：生物素是一种维生素，与亲和素和链霉亲和素（streptavidin）结合紧密。这种高亲和力使得生物素常被用于生物化学实验中的标记和分析。

马来酰亚胺-二聚乙二醇-生物素通常用于将生物素引入具有巯基的分子或表面，例如蛋白质或生物材料，以便后续的生物学实验或应用。由于其具有的特异性和稳定性，这种化合物在生物医学研究和生物技术应用中具有广泛的用途。

Maleimide-PEG2-Biotin is a compound used in bioconjugation and chemical biology. It consists of three components:

1. Maleimide: Maleimide is a reactive group that is commonly used for the covalent modification of sulfhydryl groups (-SH) in proteins or other molecules. It forms stable thioether bonds with thiol groups under mild conditions, making it useful for site-specific labeling and bioconjugation reactions.

2. PEG2 (Diethylene glycol): PEG2 is a polyethylene glycol with two repeating ethylene glycol units. It is often used as a linker to increase the solubility and flexibility of biomolecules.

3. Biotin: Biotin is a vitamin that is commonly used as a probe in various biochemical assays due to its high affinity for avidin and streptavidin.

When combined, Maleimide-PEG2-Biotin forms a versatile tool for labeling sulfhydryl-containing biomolecules, such as cysteine residues in proteins, with biotin for various applications such as affinity purification, fluorescence imaging, and drug delivery. The maleimide group reacts specifically with sulfhydryl groups to form stable covalent bonds, allowing for precise and selective labeling of target molecules.