1. Start Internet Information Services (IIS) Manager. Tip: Type “inetmgr” on the Run window then press [enter].
2. Right-click the Application Pools Node, then New, then click Application Pool.
3. In the Add New Application Pool dialog box, type TestPool in the Application Pool ID text box. Leave the Use default settings for new application pool option selected, and click OK. This creates a new application pool called TestPool.
4. You can nominate a new different username and password on your application by setting it in the Application Pool’s Properties window and go to Identity tab.
5. Return to the Internet Information Services (IIS) Manager.
6. Right-click on your web application then click Properties.
7. On the Home Directory tab in Properties dialog box, select your newly created application pool from the Application Pool drop down list. Click ok.
That’s it! Now you can run multiple versions of ASP.Net framework on your IIS 6.0.