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RSS订阅原创 Seurat PBMC教程理解分享
1.读取数据library(dplyr)library(Seurat)library(patchwork)# Load the PBMC datasetpbmc.data <- Read10X(data.dir ="../data/pbmc3k/filtered_gene_bc_matrices/hg19/")# Initialize the Seurat object with the raw (non-normalized data).pbmc <- CreateSeuratO
2021-05-23 08:41:57
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原创 R调色盘
R调色盘library(RColorBrewer)#获取颜色#获取Reds色盘所有的颜色color <- brewer.pal(9,"Reds")#分别从几个色盘中筛选颜色color <- c(brewer.pal(9,"Greens")[2:8],brewer.pal(9,"Purples"),brewer.pal(9,"RdPu")[3:4],brewer.pal(9,"Blues")[2:7],brewer.pal(9,"Greys")[3:6])...
2021-04-14 15:16:44
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翻译 Seurat常用数据处理命令
获取seuratobj的表达矩阵：counts_matrix = GetAssayData(seurat_obj, slot="counts")counts_matrix = seurat_obj@assays$RNA@counts[,colnames(seurat_obj)]获取seuratobj的metadata：meta <- myeloid.combined@meta.data获取seuratobj的子集：c0 <- subset(myeloid.combined,ide
2021-04-14 13:31:49
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