IRDye 680RD Protein Labeling Kits
IRDye 680RD Protein Labeling Kits can be used to label antibodies and other proteins for Western blotting, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section applications.
High Molecular Weight
Optimized for labeling 1.0 mg of protein with molecular weight 45 - 200 kDa.
Low Molecular Weight
Optimized for labeling 1.0 mg of protein with molecular weight 14 - 45 kDa.
IRDye 680RD 蛋白质标记试剂盒
IRDye 680RD 蛋白质标记试剂盒可用于标记抗体和其他蛋白质,用于蛋白质印迹、In-Cell Western™ 测定、体内成像以及整个器官或组织切片应用。
高分子量
针对标记 1.0 mg 分子量为 45 - 200 kDa 的蛋白质进行了优化。
低分子量
针对标记 1.0 mg 分子量为 14 - 45 kDa 的蛋白质进行了优化。
IRDye 680RD Streptavidin
IRDye 680RD Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.
IRDye 680RD 链霉亲和素
IRDye 680RD 链霉亲和素以液体形式提供,缓冲液中含有 10 mM 磷酸盐、183 mM NaCl、2.7 nM KCl,pH 7.4,叠氮化钠 0.005% (w/v) 作为防腐剂。
要使用,请在使用前短暂离心以消除可能在溶液中形成的聚集体。 这将减少非特异性背景染色。 对于大多数应用,0.2 到 1.0 µg/ml(1:1,000 到 1:5,000)的最终浓度通常是令人满意的; 但是,可能需要根据经验确定适当的稀释度。
对于基于膜的应用和 In-Gel Westerns,建议在检测孵育步骤中添加 SDS(0.02% 至 0.1% 最终浓度)和 Tween® 20(0.1 至 0.2% 最终浓度)以减少非 特定背景染色。
相关产品:
IRDye荧光标记纤维蛋白
IRDye荧光标记蛋白酶KProteinaseK
IRDye荧光标记溶菌酶Lysozyme
IRDye荧光标记Lyticase(10U/μl)溶壁酶
IRDye荧光标记蜗牛酶Snailase
IRDye荧光标记小鼠血清白蛋白MouseSerumAlbumin
IRDye荧光标记犬血清白蛋白CanineSerumAlbumin
IRDye荧光标记小麦醇溶蛋白WheatGliadin
IRDye荧光标记鬼笔环肽Phalloidin
IRDye荧光标记玉米蛋白Zein
IRDye荧光标记鱼精蛋白硫酸盐Protaminesulfatesalt
IRDye荧光标记牛血红蛋白HemoglobinfrombovinebloodHemoglobin
IRDye荧光标记γ-球蛋白γ-Globulinsfrombovineblood
IRDye荧光标记牛纤维蛋白原(进口)Fibrinogen
IRDye荧光标记血球凝集素Hemagglutinin
IRDye荧光标记Annexin膜联蛋白
IRDye荧光标记金黄色葡萄球菌蛋白A(r-SPA)
IRDye荧光标记链球菌蛋白G(r-SPG)
本文由QiyueLh整理,2022年6月7日