温度、pH和盐度对硝酸盐去除效率的影响-CSDN博客

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1.temperature

Fig.3.Effects of temperature on the removal efficiency of nitrite
	18℃		21℃		24℃		27℃		30℃		33℃
Time	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD
0d	20.26	0.31	20.22	0.04	20.20	0.11	20.26	0.35	20.26	0.35	20.21	0.33
1d	19.68	0.82	18.89	0.19	18.81	0.05	18.06	0.13	18.81	0.05	19.68	0.08
2d	19.03	0.82	16.72	0.08	16.83	0.17	15.59	0.25	16.03	0.17	18.04	0.16
3d	16.03	0.41	9.38	0.16	8.94	0.10	4.47	0.33	5.84	0.08	16.25	0.21
4d	13.17	0.22	1.65	0.17	0.65	0.11	0.05	0.01	0.05	0.00	14.36	0.17

nitrate	2.1		10.3		17.9		19.6		20.7		nd

## 2.pH

Fig.3. Effects of pH on the removal efficiency of nitrite
	pH6		pH6.5		pH7.0		pH7.5		pH8.0		pH8.5
Time	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD
0d	23.72	0.14	23.67	0.21	23.89	0.16	23.67	0.17	23.94	0.10	23.94	0.10
1d	22.82	0.08	22.84	0.05	22.90	0.00	22.82	0.00	24.05	0.08	23.91	0.05
2d	21.55	0.55	19.46	0.83	18.70	0.30	16.20	0.34	23.89	0.08	22.46	2.99
3d	19.55	0.83	11.22	0.13	8.75	0.13	5.24	0.26	23.86	0.05	23.91	0.05
4d	13.81	0.41	0.70	0.10	1.20	0.95	0.65	0.00	24.08	0.17	23.45	0.05

Nitrate	nd		19.7		21.7		21.1		nd		nd

pH	1d	2d	3d	4d	5d
6.02	6.02	5.91	5.92	5.94	6.06
	6.08	5.9	5.92	5.94	5.96
	6.03	5.96	5.96	6	5.99
6.5	6.54	6.56	6.58	6.59	6.58
	6.57	6.58	6.61	6.55	6.6
	6.49	6.5	6.54	6.6	6.58
7	6.99	7.04	7.05	7.06	7.07
	7	7.07	7.11	7.06	7.12
	7.04	7.03	7.06	7.06	7.08
7.52	7.59	7.61	7.62	7.64	7.65
	7.6	7.62	7.62	7.64	7.64
	7.6	7.61	7.63	7.64	7.65
8	8.07	8.1	8.06	8.09	8.12
	8.11	8.12	8.08	8.12	8.13
	8.11	8.1	8.09	8.12	8.12
8.5	8.44	8.5	8.4	8.44	8.45
	8.44	8.52	8.4	8.45	8.45
	8.44	8.51	8.39	8.45	8.44

3.salinity

Fig.3. Effects of salinity on the removal efficiency of nitrite
	0.13%		0.38%		0.63%		0.88%		1.13%		1.38%		1.63%
Time	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD
0d	20.47	0.05	19.88	0.09	20.55	0.09	20.55	0.09	20.47	0.05	20.53	0.05	20.53	0.05
1d	20.34	0.16	15.10	0.95	19.12	0.00	19.47	0.05	19.69	0.14	20.34	0.08	19.69	0.14
2d	20.04	0.31	7.88	0.22	18.55	0.16	19.55	0.20	19.50	0.23	20.42	0.14	20.45	0.12
3d	19.96	0.58	0.24	0.24	16.67	0.14	19.06	0.09	19.65	0.46	20.09	0.14	20.09	0.28
4d	19.71	0.05	0.00	0.00	15.81	0.12	19.96	0.09	19.71	0.09	19.61	0.08	19.50	0.17

nitrate	nd		18.5		4.4		nd		nd		nd		nd

## 4.nitrate

Fig.3. Effects of nitrate on the removal efficiency of nitrite
	0mg/L		25mg/L		50mg/L		100mg/L		200mg/L		400mg/L		800mg/L
Time	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD
0d	22.05	0.41	22.07	0.44	22.16	0.44	22.35	0.42	22.68	0.19	22.68	0.05	22.95	0.55
1d	21.80	0.34	21.41	0.49	21.63	0.38	22.27	0.52	22.68	0.17	22.65	0.30	22.76	0.25
2d	19.46	0.13	19.28	0.34	19.73	0.26	21.17	0.58	20.42	0.85	17.51	0.37	21.88	0.10
3d	17.41	0.33	18.38	0.20	17.69	0.14	17.65	0.38	17.72	0.05	18.45	0.16	21.63	0.34
4d	9.38	0.44	7.38	0.25	9.58	0.35	9.19	0.22	9.30	0.24	14.85	0.21	19.33	0.19


Nitrate	nd		35.1		47.4		44.2		nd		nd		nd

5.nitrite

Fig.3. Effects of nitrate on the removal efficiency of nitrite
	0mg/L		25mg/L		50mg/L		100mg/L		200mg/L		400mg/L		800mg/L
Time	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD
0d	22.05	0.41	22.07	0.44	22.16	0.44	22.35	0.42	22.68	0.19	22.68	0.05	22.95	0.55
1d	21.80	0.34	21.41	0.49	21.63	0.38	22.27	0.52	22.68	0.17	22.65	0.30	22.76	0.25
2d	19.46	0.13	19.28	0.34	19.73	0.26	21.17	0.58	20.42	0.85	17.51	0.37	21.88	0.10
3d	17.41	0.33	18.38	0.20	17.69	0.14	17.65	0.38	17.72	0.05	18.45	0.16	21.63	0.34
4d	9.38	0.44	7.38	0.25	9.58	0.35	9.19	0.22	9.30	0.24	14.85	0.21	19.33	0.19


Nitrate	nd		35.1		47.4		44.2		nd		nd		nd

6.optimum condition

Fig.5.Growth of the enrichment in optimum culture conditions
XY-12 5%
Time	OD600
XY-12	h-1	h-2	h-3
0d	0.002	0.003	0.000
12h	0.004	0.003	0.004
24h	0.005	0.006	0.006
36h	0.007	0.008	0.008
48h	0.014	0.014	0.012
60h	0.016	0.016	0.017
72h	0.017	0.015	0.016

Time	nitrite(mg/L)
XY-12	h-1	h-2	h-3
0d	216.87	217.68	218.09
12h	213.63	212.82	214.84
24h	207.14	208.76	209.57
36h	204.31	203.50	202.28
48h	195.80	196.61	194.99
60h	186.48	187.29	186.88
72h	166.21	169.46	166.62


Time	The total bacterial (cells/mL)
XY-12	h-1	h-2	h-3
0d	58170	48001	48740
12h	70395	136862	73053
24h	80425	123249	86457
36h	148093	137486	156552
48h	217749	264202	295170
60h	512500	589022	418364
72h	349045	726716	831636


nitrate	210

7.application

Fig 6
Concentration of NO2−–N	Freshwater shrimp water					NanHu Lake water					Brackish water (1%)
	(mg/L)	control treatment		tested treatment		(mg/L)	control treatment		tested treatment		(mg/L)	control treatment		tested treatment
	Time	mean	SD	mean	SD	Time	mean	SD	mean	SD	Time	mean	SD	mean	SD
	0d	13.49	0.20	13.31	0.07	0d	13.04	0.31	12.95	0.12	0d	13.65	0.12	13.34	0.36
	1d	13.18	0.37	11.35	0.23	1d	13.19	0.16	11.73	0.04	1d	13.73	0.16	11.96	0.18
	2d	13.72	0.16	9.64	0.24	2d	13.88	0.04	11.42	0.55	2d	13.34	0.54	11.55	0.23
	3d	13.95	0.04	9.14	0.29	3d	13.61	0.14	10.68	0.08	3d	13.35	0.26	11.73	0.10
	4d	13.95	0.23	8.13	0.55	4d	13.75	0.24	10.59	0.12	4d	13.19	0.56	11.40	0.24
	5d	13.70	0.20	5.81	0.54	5d	13.51	0.17	9.78	0.13	5d	13.84	0.04	10.24	0.06
	6d	12.97	0.22	2.22	0.18	6d	13.88	0.06	9.79	0.14	6d	12.99	0.09	9.04	0.14
	7d	12.72	0.20	0.00	0.00	7d	13.32	0.19	8.74	0.22	7d	13.22	0.10	7.22	0.18
											8d	12.24	0.13	4.77	0.12

	nitrate	nd	11.2				nd	3.7				nd	7.8

	Removal efficiency of nitrite in waters from (a) a freshwater shrimp pond, (b) NanHu Lake, and (c) brackish water

8.Enrichment

Fig. 1.	Proportion of various bacteria in the enrichment
		ref_seq	abudance
	Nitrobacter	TTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGAGTGATGAAGGCCCTAGGGTTGTAAAGCTCTTTTGTGCGGGAAGATAATGACGGTACCGCAAGAATAAGCCCCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGGTCTTTAAGTCAGAGGTGAAATCCTGGAGCTCAACTCCAGAACTGCCTTTGATACTGAGGATCTTGAGTTCGGGAGAGGTGAGTGGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAGTGGCGAAGGCGGCTCACTGGCCCGATACTGACGCTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGA	33802	99.13%
	Burkholderia	TTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATTCCGCGTGCAGGATGAAGGCCCTCGGGTTGTAAACTGCTTTTGTACGGAACGAAAAGGCTCTGGTTAATACCTGGGGCTCATGACGGTACCGTAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTGATGTAAGACAGAGGTGAAATCCCCGGGCTCAACCTGGGAACTGCCTTTGTGACTGCATCGCTGGAGTGCGGCAGAGGGGGATGGAATTCCGCGTGTAGCAGTGAAATGCGTAGATATGCGGAGGAACACCGATGGCGAAGGCAATCCCCTGGGCCTGCACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGA	123	0.36%
	Nitrosomonas	GCCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGAGTGAAGAAGGCCTTCGGGTTGTAAAGCTCTTTTAGTCGGAAAGAAAGAGTCATAGTAAATAGCTATGATTTATGACGGTACCGACAGAAAAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCCTTGTAAGTCAGATGTGAAAGCCCCGGGCTTAACCTGGGAATTGCGTTTGAAACTACAAAGCTAGAGTGCAGCAGAGGGGAGTGGAATTCCATGTGTAGCAGTGAAATGCGTAGAGATGTGGAAGAACACCGATGGCGAAGGCAGCTCCCTGGGTTGACACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCCGGTAGT	94	0.28%
	Phaseolus_acutifolius	TGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGAAGGTAGAAGGCCTACGGGTCATGAACTTCTTTTCCCGGAGAAGAAGCAATGACGGTATCCGGGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTTCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCGTAGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTGAGAGACGAAAGCTAGGGGAGCGAATGGGCTTAGATACCCCGGTAGA	49	0.14%
	Limnobacter	TTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGTGCGAAGAAGGCCTTCGGGTTGTAAAGCACTTTTGTCAGGGAAGAAATCCTTTGGGCTAATACCCTAGGGGGATGACGGTACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGTGTAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGACTAGAGTGCGTCAGAGGGGGGTGGAATTCCACGTGTAGCAGTGAAATGCGTAGATATGTGGAGGAACACCAATGGCGAAGGCAGCCCCCTGGGATGACACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGCTTAGATACCCCGGTAGA	15	0.04%
	Sphingomonas	TTACGGAAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAAGCTCTTTTACCCGGGATGATAATGACAGTACCGGGAGAATAAGCTCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGCTTTGTAAGTTAGAGGTGAAAGCCTGGAGCTCAACTCCAGAACTGCCTTTAAGACTGCATCGCTTGAATCCAGGAGAGGTGAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAGTGGCGAAGGCGGCTCACTGGACTGGTATTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGCTTAGATACCCTGGTAGA	5	0.01%
	Nocardioides	TCCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGATCCAGCAACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAACTCCGACGAAGCGAAAGTGACGGTAGGGGTAGAAGAAGGACCGGCCAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTTGTCGCGTCGGGAGTGAAAACAGCGGGCTTAACTCGTTGCTTGCTTCCGATACGGGCAGACTAGAGGTATGCAGGGGAGAATGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGTTCTCTGGGCATTACCTGACGCTGAGGAGCGAAAGTGTGGGGAGCGAACAGGATTAGATACCCGGGTAGTCCC	2	0.01%
	MWH-UniP1_aquatic_group	TTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGTGCGAAGAAGGCCTTCGGGTTGTAAAGCACTTTTGTCAGGGAAGAAATCTTTTGGGCTAATACCCCGGAAGGATGACGGTACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTCGCTAAGACAGTCGTGAAATCCCCGGGCTTAACCTGGGAACTGCGATTGTGACTGGCGAGCTAGAGTACGGCAGAGGGGGGTGGAATTCCTGGTGTAGCAGTGAAATGCGTAGAGATCAGGAGGAACACCGATGGCGAAGGCAGCCCCCTGGGCTAGTACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCGTGTAGTCCA	1	0.00%
	Ideonella	TCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGCGGGAAGAAGGCCTTCGGGTTGTAAACCGCTTTTGTCAGGGAAGAAATCTTCTGAGTTAATACCTCGGGAGGATGACGGTACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTTTGTAAGACAGAGGTGAAATCCCCGGGCTCAACCTGGGAACTGCCTTTGTGACTGCAAAGCTTGAGTGCGGCAGAGGGGGATGGAATTCCGCGTGTAGCAGTGAAATGCGTAGATATGCGGAGGAACACCGATGGCGAAGGCAATCCCCTGGGCCTGCACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCCGGTAGA	1	0.00%
	Novosphingobium	GTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAAGCTCTTTTACCAGGGATGATAATGACAGTACCTGGAGAATAAGCTCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGCTACTCAAGTCAGAGGTGAAAGCCCGGGGCTCAACCCCGGAACTGCCTTTGAAACTAGGTAGCTAGAATCTTGGAGAGGTCAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAGTGGCGAAGGCGACTGACTGGACAAGTATTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCCGGTAGA	1	0.00%
	uncultured_bacterium	ATACGGGAGGCAGCAGTAGGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGCAGGATGAATGTCCTATGGATTGTAAACTGCTTTTATATGGGAAGAAACCCCTGGATGCGTCCGGGGCTGACGGTACCATTTGAATAAGGATCGGCTAACTTCGTGCCAGCAGCCGCGGTAAGACGAAGGATTCAAGCGTTGTCCGGATTTACTGGGTTTAAAGGGTGCGTAGGCGGAAAGCTAAGTCAGTGGTGAAAGCCCGCAGCTCAACTGCGGAACTGCCATTGAAACTGGCCTTCTTGAATACAGTGGAGGTAGATGGAATATAACATGTAGCGGTGAAATGCTTAGATATGTTATAGAACACCGATTGCGAAGGCAGTCTGCTACACTGTCATTGACGCTGAGGCACGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGT	1	0.00%
	Cellvibrio	CTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGTGGGGAGAAAGGTGATTCACCTAATACGTGAGTCAATTGATGCTACCCACAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTAGGTGGTTTGTTAAGCTAGCTGTGAAATCCCCGGGCTCAACCTGGGCACTGCAGTTAGAACTGGCAAGCTAGAGTAGGGTAGAGGGGTGTGGAATTCCAGGTGTAGCGGTGAAATGCGTAGATATCTGGAGGAACATCAGTGGCGAAGGCGACACCCTGGACTCATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT	1	0.00%
	Chloroplast	ACCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGAGGGAAGAAAGCCTACTGGGTTGTAAACCTCTTTTTTCAGGGAGGAAAAAAATGACGTGTACCTGAAGAATAAGCATCGGCTAACTCCGTGCCAGCAGCCGCGGTAAGACGGAGGATGCAAGTGTTATCCGGAATTACTGGGCGTAAAGCGTCTGTAGGTGGTTTAATAAGTCAACTGTTAAATCTTGAGGCTTAACTTCAAAACAGCAGTCGAAACTATTAGACTAGAGTATAGTAGGGGTAAAGGGAATTTCCAGTGGAGCGGTGAAATGCGTAGATATTGGAAAGAACACCGATGGCGAAAGCACTTTACTGGGCTATTACTAACACTCAGAGACGAAAGCTAGGGTAGCAAATGGGATTAGATACCCTGGTAGA	1	0.00%
	hgcI_clade	TCCTACGGGACGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCGACGCCGCGTGAGGGATGAAGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAATAAGCGAAAGTGAAGGTACCTGCAGAAGAAGCACCGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGTGGTCGATCACGTCGGATGTGAAATTCTGAGGCTTAACCTCGGACCTGCATTCGATACGGGTTGACTAGAGTGTGGTAGGGGAGACTGGAATTCCTGGTGTAGCGGTGGAATGCGCAGATATCAGGAGGAACACCGATGGCGAAGGCAGGTCTCTGGGCCATTACTGACACTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGA	1	0.00%
	CHAB-XI-27	TCCTACGGGACGCAGCAGTGAGGAATCTTGGACAATGGGGGCAACCCTGATCCAGCGATGCCGCGTGTGTGAAGAAGGCCTTAGGGTTGTAAAGCACTTTAGTTGGGGAGGAGGAGTGGTTATTGAATAGATAGCCATGAGGACGTTACCCAAAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATAATTGGGCGTAAAGAGTGCGTAGGTGGTTTGTTAAGTGGTATGTGAAAGACCGGGGCTTAACCTTGGGGTGTCATATCAGACTGGCAAGCTAGAGGGCAGTAGAGGAAGGTGGAATTTCCGGTGTAGCGGTGAAATGCGTAGATATCGGGAGGAACATCAGTGGCGAAGGCGACCTTCTGGGCTGTACCTGACACTGAGGCACGAAAGCATGGGGAGCGAACAGGATTAGATACCCCGGTAGA	1	0.00%