Root microbiota shift in rice correlates with resident time in the field and developmental stage
概述
In monocots and dicots, roots mainly associate with Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes.
在单子叶和双子叶植物中，主要相关的菌群：变形菌门, 放线菌门、拟杆菌门和厚壁菌门。
实验方法
水稻种植
两品种水稻：Nipponbare水稻品种：日本晴、IR24
种子消毒：去壳，75%乙醇30s，后三次2.5%次氯酸钠15分钟。dehulled, surface sterilized in 75% ethanol for 30 s and 2.5% sodium hypochlorite three times for 15 min
接种于MS培养基发芽至2周（MS agar media）
fields on Changping farm (116.424E, 40.109N) and Shangzhuang farm (116.206E, 40.122N).
采样处理（446 samples）
对照：相应的地块的非种植土unplanted soil at
day 0 and three unplanted soil samples after 4 weeks
种下前2周：连续区间法successive intervals (0, 1, 2, 3, 5, 7, 10, and 14 days)采样，采样深度0-10cm
之后每周采样：6株Nipponbare；3株IR24，无菌水冲洗掉浮土，50ml离心管里25 mL无菌磷酸盐缓冲盐水180r
概述
In monocots and dicots, roots mainly associate with Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes.
在单子叶和双子叶植物中，主要相关的菌群：变形菌门, 放线菌门、拟杆菌门和厚壁菌门。
实验方法
水稻种植
两品种水稻：Nipponbare水稻品种：日本晴、IR24
种子消毒：去壳，75%乙醇30s，后三次2.5%次氯酸钠15分钟。dehulled, surface sterilized in 75% ethanol for 30 s and 2.5% sodium hypochlorite three times for 15 min
接种于MS培养基发芽至2周（MS agar media）
fields on Changping farm (116.424E, 40.109N) and Shangzhuang farm (116.206E, 40.122N).
采样处理（446 samples）
对照：相应的地块的非种植土unplanted soil at
day 0 and three unplanted soil samples after 4 weeks
种下前2周：连续区间法successive intervals (0, 1, 2, 3, 5, 7, 10, and 14 days)采样，采样深度0-10cm
之后每周采样：6株Nipponbare；3株IR24，无菌水冲洗掉浮土，50ml离心管里25 mL无菌磷酸盐缓冲盐水180r
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