Root microbiota shift in rice correlates with resident time in the field and developmental stage
概述
In monocots and dicots, roots mainly associate with Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes.
在单子叶和双子叶植物中,主要相关的菌群:变形菌门, 放线菌门、拟杆菌门和厚壁菌门。
实验方法
水稻种植
两品种水稻:Nipponbare水稻品种:日本晴、IR24
种子消毒:去壳,75%乙醇30s,后三次2.5%次氯酸钠15分钟。dehulled, surface sterilized in 75% ethanol for 30 s and 2.5% sodium hypochlorite three times for 15 min
接种于MS培养基发芽至2周(MS agar media)
fields on Changping farm (116.424E, 40.109N) and Shangzhuang farm (116.206E, 40.122N).
采样处理(446 samples)
对照:相应的地块的非种植土unplanted soil at
day 0 and three unplanted soil samples after 4 weeks
种下前2周:连续区间法successive intervals (0, 1, 2, 3, 5, 7, 10, and 14 days)采样,采样深度0-10cm
之后每周采样:6株Nipponbare;3株IR24,无菌水冲洗掉浮土,50ml离心管里25 mL无菌磷酸盐缓冲盐水180r
概述
In monocots and dicots, roots mainly associate with Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes.
在单子叶和双子叶植物中,主要相关的菌群:变形菌门, 放线菌门、拟杆菌门和厚壁菌门。
实验方法
水稻种植
两品种水稻:Nipponbare水稻品种:日本晴、IR24
种子消毒:去壳,75%乙醇30s,后三次2.5%次氯酸钠15分钟。dehulled, surface sterilized in 75% ethanol for 30 s and 2.5% sodium hypochlorite three times for 15 min
接种于MS培养基发芽至2周(MS agar media)
fields on Changping farm (116.424E, 40.109N) and Shangzhuang farm (116.206E, 40.122N).
采样处理(446 samples)
对照:相应的地块的非种植土unplanted soil at
day 0 and three unplanted soil samples after 4 weeks
种下前2周:连续区间法successive intervals (0, 1, 2, 3, 5, 7, 10, and 14 days)采样,采样深度0-10cm
之后每周采样:6株Nipponbare;3株IR24,无菌水冲洗掉浮土,50ml离心管里25 mL无菌磷酸盐缓冲盐水180r