Description
NOVOPROTEIN Recombinant Human CD3D&CD3E is produced by our Mammalian expression system and the target gene encoding Phe22-Ala105&Asp23-Asp126 is expressed
**Accession #😗*P04234&P07766
Known as:CD3E & CD3D; CD3 delta & CD3 epsilon; CD3 delta / epsilon
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
Quality Control
**Purity:**Greater than 95% as determined by reducing SDS-PAGE.
**Endotoxin:**Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.