25-羟基维生素D酶联免疫检测试剂盒 25(OH) Vitamin D ELISA kit

最近的研究表明，维生素D水平影响各种疾病状态，并与人类健康的许多指标相关联。这些疾病包括骨质疏松症和关节炎等骨骼疾病，也包括高血压、糖尿病、癌症和心脏病等其他疾病。Enzo Life Sciences的25-羟基维生素D ELISA试剂盒为快速便捷检测人血浆和血清中25-羟基维生素D水平提供了一种方法。

维生素D向活性形式的转化始于7-脱氢胆固醇被紫外线作用形成母体维生素D3。或者来源于食物摄取的母体维生素D2。这些母体化合物被运输到肝脏并羟基化为25(OH)维生素D。这种代谢物随后被运输到肾脏，再次羟基化为1，25(OH)2维生素D，这是维生素D2的生物活性形式。维生素D代谢物的水平随着母体维生素D摄取量的增加而成比例增加。与活性形式相比，25(OH)维生素D在循环中的半衰期和稳定性更长，这是25(OH)维生素D代谢物的检测被用作总维生素D浓度指标的原因。 

产品信息

产品货号	ADI-900-215-0001
产品名称	25(OH) Vitamin D ELISA kit / 欣博盛生物
规格	96 wells
灵敏度	1.98 ng/ml
检测范围	0.5-1010 ng/ml
其他名称	25-Hydroxyvitamin D
检测时间	~1.5 hours
适用样本类型	血清，血浆
适用种属	人
交叉反应	25(OH) Vitamin D3 (100%), 25(OH) Vitamin D2 (~80%), 1,25(OH)2-Vit. D2 (>100%), 1,25(OH)2-Vitamin D3 (>100%),  3-epi 25(OH) Vitamin D3 (~10%), 24,25(OH)2-Vitamin D3 (~6%) and <1%: 1α(OH) Vitamin D2, 1α(OH) Vitamin D3,  Vitamin D2, Vitamin D3
试剂盒组分	Microtiter Plate, Conjugate Concentrate, Antibody, Dissociation Buffer, Conjugate Diluent, Sample Diluent, Wash Buffer  Concentrate, Standards, Substrate, Stop Solution, Assay Layout

标准曲线示例

部分产品文献引用

1. Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf. 225, 112771 (2021)

2. Vitamin K and D Status in Patients with Knee Osteoarthritis: An Analytical Cross-sectional Study: A. Askari, et al.; Mediterr. J. Rheumatol. 32, 350 (2021)

3. Compounding effect of vitamin D3 diet, supplementation, and alcohol exposure on macrophage response to mycobacterium infection: M.E. Gough, et al.; Tuberculosis (Edinb.) 116S, S42 (2019), Application(s): ELISA using culture supernatants

4. VDR gene methylation as a molecular adaption to light exposure: Historic, recent and genetic influences: E.L. Beckett, et al.; Am. J. Hum. Biol. 29, (2017)

5. A study of Vitamin D Levels in hypothyroid patients, a case control study in a tertiary care hospital of central India: R. Kohokare, et al.; NJMDR 4, 89 (2016), Application(s): Vitamin D levels were measured from serum of hypothyroid patients

6. Evaluation of vitamin D relationship with type 2 diabetes and systolic blood pressure: S.B. Nayak, et al.; BMJ Open Diabetes Res. Care 4, e000285 (2016), Application(s): Determination of 25(OH)D levels in human plasma and whole blood samples

7. Reduced 25-OH vitamin D in patients with autoimmune cytopenias, clinical correlations and literature review: B. Fattizzo, et al.; Autoimmun. Rev. 15, 770 (2016), Application(s): Measured Vitamin D in 40 year old humans

8. Relationship between methylation status of vitamin D-related genes, vitamin D levels, and methyl-donor biochemistry: E.L. Beckett, et al.; J. Nutr. Int. Metab. 6, 8 (2016), Application(s): ELISA using human plasma,

9. Vitamin D deficiency is associated with endothelial dysfunction and increases type I interferon gene expression in a murine model of systemic lupus erythematosus: J.A. Reynolds, et al.; Arthritis Rheumatol. 68, 2929 (2016)

10. Vitamin D status in different stages of disease severity in dogs with chronic valvular heart disease: T. Osuga, et al.; J. Vet. Intern. Med. 29, 1518 (2015), Application(s): ELISA using dog serum