反卷积算法

Deconvolution

All microscopes are limited by the laws of physics, and these laws state that when light passes through a medium, that light will bend. This is one of the most common causes of haze and blur in microscopy images. Deconvolution can correct this problem, not only removing the haze and blur, but restoring vital detail to datasets.  This software can be used to sharpen fluorescence, brightfield, darkfield, confocal, ESM, DIC, and many other kinds of images.

Penicillum Chrysogenum stained with FUN1Viability kit; original and deconvolved images.

 

E. coli stained with Live/Dead kit; original and deconvolved images.

 

来源：http://web.utk.edu/~jbiggers/Deconvolution.html

GFP Tubulin in mouse fibroblasts, DAPI stained nuclei; original and deconvolved images.