FullSSR.pl运行报错。

最新推荐文章于 2024-07-10 17:50:59 发布

Henrietta_L

最新推荐文章于 2024-07-10 17:50:59 发布

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 ./FullSSR.pl
Can't locate Bio/Tools/Run/Primer3.pm in @INC (@INC contains: /root/perl5/lib/perl5/x86_64-linux-thread-multi /root/perl5/lib/perl5 /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at ./FullSSR.pl line 8.
BEGIN failed--compilation aborted at ./FullSSR.pl line 8.






./FullSSR.pl
 
    Execute: FullSSR.pl [parameters]
 
		--i   : input file name (obligatory)
		--m   : minimum repeat of motifs (optional, defoult 4 minimum repeat)
		--h   : help
 




./FullSSR.pl -i /home/112shiyanshi/下载/FullSSR/Example/sequences.fasta -m 5

------------- EXCEPTION: Bio::Root::Exception -------------
MSG: Cannot find executable for primer3_core. path="/home/../primer3-2.3.6/src/primer3_core"
STACK: Error::throw
STACK: Bio::Root::Root::throw /root/perl5/lib/perl5/Bio/Root/Root.pm:449
STACK: Bio::Tools::Run::WrapperBase::executable /root/perl5/lib/perl5/Bio/Tools/Run/WrapperBase.pm:340
STACK: ./FullSSR.pl:71
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