安装get_homologues

官方网址

https://omictools.com/get-homologues-tool

帮助文档

https://github.com/eead-csic-compbio/get_homologues/blob/master/manual_get_homologues.pdf

软件安装步骤

1 软件获取

wget https://github-cloud.s3.amazonaws.com/releases/46931871/af7a24f0-240a-11e6-8c41-e87b0ee2ed8d.tgz?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAISTNZFOVBIJMK3TQ%2F20160622%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Date=20160622T142013Z&X-Amz-Expires=300&X-Amz-Signature=672fd831dd3147c8bbb843b5d1dbd2de9b7144cd5756069f48342bf69a4d5170&X-Amz-SignedHeaders=host&actor_id=0&response-content-disposition=attachment%3B%20filename%3Dget_homologues-x86_64-20160527.tgz&response-content-type=application%2Foctet-stream

2 解压

tar zxvf get_homologues-2.0.16.tar.gz

3 阅读README文件

注意:所有依赖软件均需安装至’get_homologues-2.0.16/bin’目录下。

依赖软件

1 COGtriangles(COGsoft)
2 hmmer(hmmer-3.1b2)
3 MCL(mcl-14-137)
4 BLAST(ncbi-blast-2.2.27+)

4 修改依赖软件文件夹名称

mv COGsoft.04.19.2012/ COGsoft
mv hmmer-3.1b2-linux-intel-x86_64/ hmmer-3.1b2
mv mcl-14-137* mcl-14-137
mv ncbi-blast-2.2.27+*/ ncbi-blast-2.2.27+

5 安装get_homologues

cd get_homologues-2.0.16
perl install.pl

安装截图:
安装截图1
安装截图2

安装过程中会提示下载两个依赖软件(Pfam-A,SWISSPROT),选择‘y’即可。

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精简下面表达:Existing protein function prediction methods integrate PPI networks and multivariate bioinformatics data to improve the performance of function prediction. By combining multivariate information, the interactions between proteins become diverse. Different interactions’ functions in functional prediction are various. Combining multiple interactions simply between two proteins can effectively reduce the effect of false negatives and increase the number of predicted functions, but it can also increase the number of false positive functions, which contribute to nonobvious enhancement for the overall functional prediction performance. In this article, we have presented a framework for protein function prediction algorithms based on PPI network and semantic similarity with the addition of protein hierarchical functions to them. The framework relies on diverse clustering algorithms and the calculation of protein semantic similarity for protein function prediction. Classification and similarity calculations for protein pairs clustered by the functional feature are more accurate and reliable, allowing for the prediction of protein function at different functional levels from different proteomes, and giving biological applications greater flexibility.The method proposed in this paper performs well on protein data from wine yeast cells, but how well it matches other data remains to be verified. Yet until now, most unknown proteins have only been able to predict protein function by calculating similarities to their homologues. The predictions result of those unknown proteins without homologues are unstable because they are relatively isolated in the protein interaction network. It is difficult to find one protein with high similarity. In the framework proposed in this article, the number of features selected after clustering and the number of protein features selected for each functional layer has a significant impact on the accuracy of subsequent functional predictions. Therefore, when making feature selection, it is necessary to select as many functional features as possible that are important for the whole interaction network. When an incorrect feature was selected, the prediction results will be somewhat different from the actual function. Thus as a whole, the method proposed in this article has improved the accuracy of protein function prediction based on the PPI network method to a certain extent and reduces the probability of false positive prediction results.
最新发布
02-27
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