Quality trimming using Trimmomatic

最新推荐文章于 2024-10-06 20:16:20 发布

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最新推荐文章于 2024-10-06 20:16:20 发布

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文章标签： java

原文链接：http://www.cnblogs.com/liuhui0622/p/4689910.html

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java -jar /home/liuhui/bin/trinityrnaseq_r20140413p1/trinity-plugins/Trimmomatic/trimmomatic.jar PE -threads 20 -phred33 P_ACAGTG_L002_R1_001.fastq.gz P_ACAGTG_L002_R2_001.fastq.gz \
P_ACAGTG_L002_R1_001.fastq.gz.P.qtrim P_ACAGTG_L002_R1_001.fastq.gz.U.qtrim P_ACAGTG_L002_R2_001.fastq.gz.P.qtrim P_ACAGTG_L002_R2_001.fastq.gz.U.qtrim SLIDINGWINDOW:4:5 LEADING:5 TRAILING:5 MINLEN:25

cat P_ACAGTG_L002_R1_001.fastq.gz.P.qtrim P_ACAGTG_L002_R1_001.fastq.gz.U.qtrim > P_ACAGTG_L002_R1_001.fastq.gz.PwU.qtrim.fq
cat P_ACAGTG_L002_R2_001.fastq.gz.P.qtrim P_ACAGTG_L002_R2_001.fastq.gz.U.qtrim > P_ACAGTG_L002_R2_001.fastq.gz.PwU.qtrim.fq

gzip P_ACAGTG_L002_R1_001.fastq.gz.P.qtrim P_ACAGTG_L002_R1_001.fastq.gz.U.qtrim P_ACAGTG_L002_R2_001.fastq.gz.P.qtrim P_ACAGTG_L002_R2_001.fastq.gz.U.qtrim &

转载于:https://www.cnblogs.com/liuhui0622/p/4689910.html