Epigenetic contribution to successful polyploidizations: variation in global cytosine methylation al...

These days, I was busing in learning Russian language and I will have a exam in half month. I also have classes in the department of ecology every Wednesday. That is to say, there are not many time for me to do my research. I have to make good use of my precious time to read latest paper, analyses my upset data and write my scientific paper.

I want to write a SCI paper in English and look forward to working for a PHD in USA in the future. In order to improve my English skills, I decide to write my blogs in English. It must be difficult for to make it, but I have to do it. Today, let me start with this rapid report published by New Phytologist, which is a excellent journal with IF more than 7.

The citation is following:

Alonso C., Balao F., Bazaga P. & Pérez R. 2016. Epigenetic contribution to successful polyploidizations: variation in global cytosine methylation along an extensive ploidy series in Dianthus broteri (Caryophyllaceae). New Phytol. 212: 571–576. 

This research resemble one of my interested work about reed, which I consider as good materials for study on the epigenetic changes of polyloidization .However, my work used MSAP instead of HPLC.

As it is so late, let’s see the summary only:

• Polyploidization is a significant evolutionary force in plants which involves major genomic and genetic changes, frequently regulated by epigenetic factors. We explored whether natural polyploidization in Dianthus broteri complex resulted in substantial changes in global DNA cytosine methylation associated to ploidy.  // Introduciton:Polyploidization is important in evolution and revolted in epigenetics.
• Global cytosine methylation was estimated by high-performance liquid chromatography (HPLC) in 12 monocytotypic populations with different ploidies (2×, 4×, 6×, 12×) broadly distributed within D. broteri distribution range. The effects of ploidy level and local variation on methylation were assessed by generalized linear mixed models (GLMMs).  // Material and method : HPLC with different ploidies  
• Dianthus broteri exhibited a higher methylation percent (˜33%) than expected by its monoploid genome size and a large variation among study populations (range: 29.3–35.3%). Global methylation tended to increase with ploidy but did not significantly differ across levels due to increased variation within the highest-order polyploidy categories. Methylation varied more among hexaploid and dodecaploid populations, despite such cytotypes showing more restricted geographic location and increased genetic relatedness than diploids and tetraploids. //Result: variation whih and within populaitons.
• In this study, we demonstrate the usefulness of an HPLC method in providing precise and genome reference-free global measure of DNA cytosine methylation, suitable to advance current knowledge of the roles of this epigenetic mechanism in polyploidization processes. //Discussion:  HPLC is a feasible method.

 

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