近红外荧光染料IRDye 700 AP-1/CREB/HIF-1/NFĸB/p53/Sp-1/STAT3 Consensus Oligonucleotide共有寡核苷酸

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最新推荐文章于 2024-10-14 14:51:34 发布

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　　IRDye 700 AP-1 Consensus Oligonucleotide

　　IRDye 700 AP-1 共有寡核苷酸

5′ -- CGC TTG ATG ACT CAG CCG GAA -- 3′

　　3′ -- GCG AAC TAC TGA GTC GGC CTT -- 5′

　　Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.

　　AP-1 is a transcription factor that regulates gene expression and controls a number of cellular processes including differentiation, proliferation, and apoptosis. For this reason, it is a common oligo used in EMSA to assess protein binding.

　　Since IRDye infrared dyes are somewhat sensitive to light, it is best to keep binding reactions in the dark during incubation periods (e.g., put the tubes into a drawer or simply cover the rack containing tubes with aluminum foil). After the incubation period, 10X Orange Loading Dye (P/N 927-10100) is added to the binding reaction for electrophoresis.

　　5' -- CGC TTG ATG ACT CAG CCG GAA -- 3'

　　3' -- GCG AAC TAC TGA GTC GGC CTT -- 5'

　　带下划线的核苷酸是结合位点。 IRDye 700 寡核苷酸以 25 µL 的 50 nM(或 50 fmol/µL)双链 DNA 形式提供。

　　AP-1 是一种转录因子，可调节基因表达并控制许多细胞过程，包括分化、增殖和凋亡。出于这个原因，它是 EMSA 中用于评估蛋白质结合的常见寡核苷酸。

　　由于 IRDye 红外染料对光有些敏感，因此最好在孵育期间将结合反应保持在黑暗中(例如，将试管放入抽屉或简单地用铝箔盖住装有试管的架子)。孵育期后，将 10X Orange Loading Dye (P/N 927-10100) 添加到结合反应中进行电泳。

　　IRDye 700 CREB Consensus Oligonucleotide

　　IRDye 700 CREB 共有寡核苷酸

　　5' -- AGA GAT TGC CTG ACG TCA GAG AGC 标记 -- 3'

　　3' -- TCT CTA ACG GAC TGC AGT CTC TCG ATC -- 5'

　　带下划线的核苷酸是结合位点。 IRDye 700 寡核苷酸以 25 µL 的 50 nM(或 50 fmol/µL)双链 DNA 形式提供。

　　CREB 蛋白是由与某些 DNA 序列结合的各种激酶的磷酸化激活的细胞转录因子。出于这个原因，它是 EMSA 中用于评估蛋白质结合的常见寡核苷酸。

　　将 DNA 添加到蛋白质缓冲液混合物中后，孵育反应物以使蛋白质与 DNA 结合。典型的孵育条件是室温下 20-30 分钟。

　　5′ -- AGA GAT TGC CTG ACG TCA GAG AGC TAG -- 3′

　　3′ -- TCT CTA ACG GAC TGC AGT CTC TCG ATC -- 5′

　　Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.

　　CREB proteins are cellular transcription factors activated by phosphorylation of various kinases that bind to certain DNA sequences. For this reason, it is a common oligonucleotide used in EMSA to assess protein binding.

　　After the addition of the DNA to the protein-buffer mix, reactions are incubated to allow protein binding to DNA. A typical incubation condition is 20-30 minutes at room temperature.

　　IRDye 700 HIF-1 Consensus Oligonucleotide

　　IRDye 700 HIF-1 共有寡核苷酸

　　5' -- AGC TTG CCC TAC GTG CTG TCT CAG A -- 3'

　　3' -- TCG AAC GGG ATG CAC GAC AGA GTC T -- 5'

　　带下划线的核苷酸是结合位点。 IRDye 700 寡核苷酸以 25 µL 的 50 nM(或 50 fmol/µL)双链 DNA 形式提供。

　　HIF-1 是一种转录因子，可调节细胞对缺氧的反应，这与癌症生物学和各种其他病理生理学密切相关。出于这个原因，它是基础发现和治疗研究中 EMSA 检测中常用的寡核苷酸。

　　将 DNA 添加到蛋白质缓冲液混合物中后，孵育反应物以使蛋白质与 DNA 结合。典型的孵育条件是室温下 20-30 分钟。

　　5′ -- AGC TTG CCC TAC GTG CTG TCT CAG A -- 3′

　　3′ -- TCG AAC GGG ATG CAC GAC AGA GTC T -- 5′

　　Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.

　　HIF-1 is a transcription factor that regulates celluar responses to hypoxia, which have been heavily implicated in cancer biology and various other pathophysiologies. For this reason, it is a common oligonucleotide used in EMSA assays in basic discovery and therapeutics research.

　　After the addition of the DNA to the protein-buffer mix, reactions are incubated to allow protein binding to DNA. A typical incubation condition is 20-30 minutes at room temperature.

　　IRDye 700 NFĸB Consensus Oligonucleotide

　　IRDye 700 NFĸB 共有寡核苷酸

　　5' -- AGT TGA GGG GAC TTT CCC AGG C -- 3'

　　3' -- TCA ACT CCC CTG AAA GGG TCC G -- 5'

　　带下划线的核苷酸是结合位点。 IRDye 700 寡核苷酸以 25 µL 的 50 nM(或 50 fmol/µL)双链 DNA 形式提供。

　　NFκB 是一种转录因子，可控制 DNA 转录、细胞因子产生和细胞存活。出于这个原因，它是 EMSA 中用于评估特定蛋白质结合的常见寡核苷酸。

　　将 DNA 添加到蛋白质缓冲液混合物中后，孵育反应物以使蛋白质与 DNA 结合。典型的孵育条件是室温下 20-30 分钟。

　　5′ -- AGT TGA GGG GAC TTT CCC AGG C -- 3′

　　3′ -- TCA ACT CCC CTG AAA GGG TCC G -- 5′

　　Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.

　　NFκB is a transcription factor that controls transcription of DNA, cytokine production, and cell survival. For this reason, it is a common oligonucleotide used in EMSA to assess the binding of specific proteins.

　　After the addition of the DNA to the protein-buffer mix, reactions are incubated to allow protein binding to DNA. A typical incubation condition is 20-30 minutes at room temperature.

　　IRDye 700 p53 Consensus Oligonucleotide

　　IRDye 700 p53 共有寡核苷酸

　　5' -- TAC AGA ACA TGT CTA AGC ATG CTG GGG ACT -- 3'

　　3' -- ATG TCT TGT ACA GAT TCG TAC GAC CCC TGA -- 5'

　　带下划线的核苷酸是结合位点。 IRDye 700 寡核苷酸以 25 µL 的 50 nM(或 50 fmol/µL)双链 DNA 形式提供。

　　p53 是一种细胞肿瘤抗原，起肿瘤抑制作用。因此，它是 EMSA 中常用的寡核苷酸，用于评估癌症和相关研究中的蛋白质结合

　　将 DNA 添加到蛋白质缓冲液混合物中后，孵育反应物以使蛋白质与 DNA 结合。典型的孵育条件是室温下 20-30 分钟。

　　5′ -- TAC AGA ACA TGT CTA AGC ATG CTG GGG ACT -- 3′

　　3′ -- ATG TCT TGT ACA GAT TCG TAC GAC CCC TGA -- 5′

　　Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.

　　p53 is a cellular tumor antigen that functions as a tumor suppresor. For this reason, it is a common oligonucleotide used in EMSA to assess protein binding in cancer and related research

　　After the addition of the DNA to the protein-buffer mix, reactions are incubated to allow protein binding to DNA. A typical incubation condition is 20-30 minutes at room temperature.

　　IRDye 700 Sp-1 Consensus Oligonucleotide

　　IRDye 700 Sp-1 共有寡核苷酸

　　5' -- ATT CGA TCG GGG CGG GGC GAG C -- 3'

　　3' -- TAA GCT AGC CCC GCC CCG CTC G -- 5'

　　带下划线的核苷酸是结合位点。 IRDye 700 寡核苷酸以 25 µL 的 50 nM(或 50 fmol/µL)双链 DNA 形式提供。

　　Sp-1 是一种转录因子，可调节基因表达并控制许多细胞过程，包括分化、增殖和凋亡。出于这个原因，它是 EMSA 中用于评估某些类型的蛋白质结合的常见寡核苷酸。

　　将 DNA 添加到蛋白质缓冲液混合物中后，孵育反应物以使蛋白质与 DNA 结合。典型的孵育条件是室温下 20-30 分钟。

　　5′ -- ATT CGA TCG GGG CGG GGC GAG C -- 3′

　　3′ -- TAA GCT AGC CCC GCC CCG CTC G -- 5′

　　Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.

　　Sp-1 is a transcription factor that regulates gene expression and controls a number of cellular processes including differentiation, proliferation, and apoptosis. For this reason, it is a common oligonucleotide used in EMSA to assess certain types protein binding.

　　After the addition of the DNA to the protein-buffer mix, reactions are incubated to allow protein binding to DNA. A typical incubation condition is 20-30 minutes at room temperature.

　　IRDye 700 STAT3 Consensus Oligonucleotide

　　IRDye 700 STAT3 共有寡核苷酸

　　5' -- GAT CCT TCT GGG AAT TCC TAG ATC -- 3'

　　3' -- CTA GGA AGA CCC TTA AGG ATC 标记 -- 5'

　　带下划线的核苷酸是结合位点。 IRDye 700 寡核苷酸以 25 µL 的 50 nM(或 50 fmol/µL)双链 DNA 形式提供。

　　STAT3 是一种转录因子，它通过酪氨酸 705 的磷酸化并响应特定的配体而被激活。出于这个原因，它是 EMSA 中用于评估某些类型的蛋白质结合的常见寡核苷酸。

　　5′ -- GAT CCT TCT GGG AAT TCC TAG ATC -- 3′

　　3′ -- CTA GGA AGA CCC TTA AGG ATC TAG -- 5′

　　Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.

　　STAT3 is a transcription factor that is activated by phosphorylation of tyrosine 705 and in response to particular ligands. For this reason, it is a common oligonucleotide used in EMSA to assess certain types of protein binding.