Good primer design is essential for a successful PCR reaction. There are many factors to take into account when designing the optimal primers for your gene of interest. Here are some tips to consider when designing primers.
- In general, a length of 18–30 nucleotides for primers is good.引物长度
- Try to make the melting temperature (Tm) of the primers between 65°C and 75°C, and within 5°C of
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