读《茶树冷胁迫的全局基因表达谱》

最新推荐文章于 2024-05-29 21:45:00 发布

SicongFu

最新推荐文章于 2024-05-29 21:45:00 发布

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文章标签：二代测序

本文链接：https://blog.csdn.net/sicongfu/article/details/51517565

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本文通过二代测序技术分析了茶树在冷驯化过程中的全局基因表达变化。研究发现，约有1770个基因表达差异，其中1168个上调，602个下调，涉及寒冷传感、信号转导、细胞膜稳定等关键过程。通路分析表明，碳水化合物代谢途径和钙信号通路可能在茶树应对低温胁迫中起重要作用。

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Global transcriptome profiles ofCamellia sinensis

during cold acclimation

文章第一部分：背景、实验结果、结论、关键词

背景：茶多么多么重要。。。在冬天，茶需要经历低温去提高自身的抗冻能力。低温中分子水平上发生的变化还没有被广泛研究。为了阐明低温胁迫的分子机理，我们使用了转录组（RNA-seq）和数字表达谱digital gene expression(DGE)技术来低温过程中基因表达谱。

实验结果：五千多万条RNA-Seq reads——assembled into 216,831transcripts，with an average length of 356 bp and an N50 of 529 bp
——1,770 differentially expressed transcripts were identified, of which 1,168 were up-regulated and 602 down-regulated
——这其中包括：寒冷传感相关、信号转导基因、冷敏感转录因子基因、细胞膜稳定相关基因、osmosensing 响应、解毒酶基因。

DGE和荧光定量PCR分析证实了来自转录组的结果是正确的。通路分析：carbohydrate metabolism pathway、calcium signaling pathway
might play a vital role in tea plants’ responses to cold stress

结论：a global survey of transcriptome profiles of tea plants in response to low,non-freezing temperatures and yields insights into the molecular mechanisms of tea plants during the cold acclimation process

Keywords: Camellia Sinensis, Cold Acclimation, RNA-Seq, DGE, Genome-wide Expression Profiles, Tea Plants

下面是正文内容：

cold acclimation (CA)

When plants sense the cold temperature, a series of protective mechanisms are triggered.

当植物感受到低温时，一系列自我保护的生物机制被触发。

A group of cold-related genes has been reported to regulate these aforementioned changes.

一群低温相关的基因已经被报道与上述这些变化的调节有关。

Moreover, changes in geneexpression have been demonstrated to occur during CA in a wide range of plant species, and hundreds of cold inducible genes have been identified
而且，

A previous study showed that when the aver