5-TAMRA-Beta-Amyloid (1-40)序列信息:
序列(单字母): TAMRA-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV
序列(三字母): TAMRA-Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-OH
CAS No.: 1802087-81-5
分子式: C219H315N55O62S
分子量: 4742.61
结构图:
5-TAMRA-Beta-Amyloid (1-40)是Aβ40的N端标记了TAMRA荧光染料,Anderson 和 Webb 通过透射电镜证实对Aβ40 N端标记TAMRA和其他荧光染料并不会阻止原纤维和amyloid纤维质的形成。
文章:Transmission electron microscopy characterization of fluorescently labelled amyloid β 1-40 and α-synuclein aggregates
作者:Valerie L Anderson & Watt W Webb
期刊:BMC Biotechnology, Volume 11, article number 125, (2011)
Abstract
Fluorescent tags, including small organic molecules and fluorescent proteins, enable the localization of protein molecules in biomedical research experiments. However, the use of these labels may interfere with the formation of larger-scale protein structures such as amyloid aggregates. Therefore, we investigate the effects of some commonly used fluorescent tags on the morphologies of fibrils grown from the Alzheimer's disease-associated peptide Amyloid β 1-40 (Aβ40) and the Parkinson's disease-associated protein α-synuclein (αS).
Results
Using transmission electron microscopy (TEM), we verify that N-terminal labeling of Aβ40 with AMCA, TAMRA, and Hilyte-Fluor 488 tags does not prevent the formation of protofibrils and amyloid fibrils of various widths. We also measure the two-photon action cross-section of Aβ40 labelled with Hilyte Fluor 488 and demonstrate that this tag is suitable for use with two-photon fluorescence techniques. Similarly, we find that Alexa Fluor 488 labelling of αS variant proteins near either the N or C terminus (position 9 or 130) does not interfere with the formation of amyloid and other types of αS fibrils. We also present TEM images of fibrils grown from αS C-terminally labelled with enhanced green fluorescent protein (EGFP). Near neutral pH, two types of αS-EGFP fibrils are observed via TEM, while denaturation of the EGFP tag leads to the formation of additional species.
使用透射电子显微镜(TEM),我们验证了用AMCA、TAMRA和Hilyte Fluor 488标签对Aβ40的N端标记不会阻止不同宽度的原纤维和淀粉样纤维的形成。我们还测量了用Hilyte Fluor 488标记的Aβ40的双光子作用截面,并证明该标签适用于双光子荧光技术。类似地,我们发现Alexa Fluor 488标记N或C末端附近的αS变体蛋白(位置9或130)不会干扰淀粉样蛋白和其他类型的αS原纤维的形成。我们还展示了用增强型绿色荧光蛋白(EGFP)标记的αS C-末端生长的原纤维的TEM图像。在接近中性pH的情况下,通过TEM观察到两种类型的αS-EGFP原纤维,而EGFP标签的变性导致额外物种的形成。
产品链接:http://www.genicbio.cn/detail.html?article_id=87
上海捷妮泰生物科技有限公司(GenicBio Limited)
微 信: 199 1653 7972
Q Q: 1704635669
邮 箱: service@genicbio.cn
网 址: www.genicbio.cn