梁颖
1,
裴瑾
2*,
刘薇
2,
万德光
2
(
1、
成都中医药大学 中药材标准化教育部重点实验室;
2、
成都中医药大学 中药材标准化教育部重点实验室,四川 成都 610075; )
摘要:目的 研究纤维素酶提取红花黄色素( safflow yellow, SY)的提取工艺条件。方法 以红花黄色素和羟基红花黄色素A的提取量为评价指标,采用单因素实验法和正交实验法考察纤维素酶、溶剂用量、提取时间、提取温度等因素对红花黄色素提取量的影响。结果 以羟基红花黄色素A为评价指标,其最佳提取工艺为:溶媒PH值为5.5、提取温度为60℃、液料比为80﹕1、料酶比为400﹕1、提取时间50min、提取次数为两次。结论 该工艺红花黄色素的提取量最高,可用于红花黄色素的提取。
关键词:红花黄色素;羟基红花黄色素A;提取;纤维素酶
关键词:红花黄色素;羟基红花黄色素A;提取;纤维素酶
LIANG Ying
1,
PEI Jin
2*,
LIU Wei
2,
WAN Deguang
2
(
1、
Education Ministry Key Laboratory for standardization of TCM, Chengdu University of Traditional Chinese Medicine;
2、
Education Ministry Key Laboratory for standardization of TCM, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China; )
Abstract: Objective:Cellulase of safflower yellow pigment extraction safflow (safflow yellow, SY). Methods:With safflower yellow and hydroxyl safflower yellow A for index, using single factor test and the orthogonal test, solvent extraction of cellulose enzyme, time, temperature of safflower yellow pigment extraction. Results:With hydroxyl safflower yellow A , the optimum extraction solvent PH: for 5.5, extraction temperature for 60 degrees, the liquid ratio of 80:1, 1:4 for enzyme. Conclusion:The process of safflower yellow pigment extraction of safflower yellow pigment, can be used for the extraction.
Keywords:Safflower yellow;Hydroxyl safflower yellow A;Extraction;Hellulose enzyme
Keywords:Safflower yellow;Hydroxyl safflower yellow A;Extraction;Hellulose enzyme