25-羟基胆固醇通过p53 / SREBF2/NFYA 信号传导途径下调氧化固醇结合蛋白2（OSBPL2）

25-hydroxycholesterol down-regulates oxysterol binding protein like 2 (OSBPL2) via the p53 / SREBF2 / NFYAA signaling pathway.

人体抑癌基因/固醇调节因子结合蛋白2 

Oxysterol Binding Protein Like 2 (OSBPL2) is a lipid-binding protein implicated in various cellular processes. Previous studies have shown that depression of OSBPL2 significantly increases the level of cellular 25-hydroxycholesterol (25-OHC) which regulates the expression of lipid-metabolism-related genes. However, whether 25-OHC can regulate the expression of OSBPL2 remains unanswered. This study aimed to explore the molecular mechanism of 25-OHC regulating the expression of OSBPL2. Using dual-luciferase reporter assay, we found a decrease of nuclear transcription factor Y subunit alpha (NFYA) bound with OSBPL2 promoter when HeLa cells were treated with 25-OHC. Furthermore, transcriptome sequencing and RNA interference results revealed that the p53/sterol regulatory element binding transcription factor 2 (SREBF2) signal