截屏脚本

using System.Collections;
using UnityEngine;
using System.IO;

public class printScreen : MonoBehaviour {
    private Texture2D screenShot;
    private bool shoot = false;
	void Start () {
        screenShot = new Texture2D(256, 256, TextureFormat.RGB24, false);
	}
	void Update () {
        if (Input.GetKeyUp(KeyCode.Mouse0)) {
            StartCoroutine(CaptureScreenshot());
        }
	}
    void OnGUI() {
        if (shoot) {
            GUI.DrawTexture(new Rect(10, 10, 256, 256), 
                screenShot, ScaleMode.StretchToFill);
        }
    }
    IEnumerator CaptureScreenshot() {
        yield return new WaitForEndOfFrame();

        screenShot.ReadPixels(new Rect(0, 0, Screen.width, Screen.height), 0, 0);
        screenShot.Apply();

        byte[] bytes = screenShot.EncodeToPNG();
        string filename = Application.dataPath + "/" + Time.time.ToString() + ".png";
        Debug.Log(Application.dataPath);
        File.WriteAllBytes(filename, bytes);
        shoot = true;
    }
}

小测试：在场景里随便添加几个方块，然后把脚本挂在相机上，运行场景

截图的位置是从屏幕的左下角开始的，如果想从中心截屏，把 ReadPixels(new Rect(0,0,Screen.width,Screen.height),0,0) 改为 ReadPixels(new Rect(Screen.width*0.5f,Screen.height*0.5f,256,256),0,0) 然后就可以了。

ReadPixels函数中的第一个参数理解为：读取的屏幕像素矩形区域是以game视窗的左下角为原点的矩形区域。第二、第三个参数是：位移参数一的偏移量，向左或向右，向上或向下 偏移。