课题设计避坑指南

实验设计

样本分组的不均一性

• case vs control 一般数量要求1：1
  A large number of subjects was included in the study. However, sample size for each group is uneven, which could potentially biases the results (62 healthy subjects and 33 dandruff subjects). 1. 研究中包括了大量的受试者。然而，每组的样本量是不均匀的，这有可能使结果出现偏差（62名健康受试者和33名头皮屑受试者）。
  
  Longitudinal study of the scalp microbiome suggests coconut oil to enrich healthy scalp commensals

样本采集方法与前人不同

An unconventional sampling method was used (line 465), which could interfere negatively with the results. It is not possible to know where exactly microorganisms are from (scalp, hair). Moreover, it depends on hair length and texture, which probably vary among subjects. 使用了非常规的取样方法（第465行），这可能会对结果产生负面的干扰。不可能确切知道微生物来自哪里（头皮、头发）。此外，这取决于头发的长度和质地，而这些可能在受试者之间有所不同。

样本污染问题

• 实验必须设置阴性对照
  Sterile cotton swabs not exposed to skin surfaces were included in the extraction process as negative controls to account for possible contamination. Purified gDNA samples, including negative controls, were sent to Health Genetech Corporation (Taoyuan City, Taiwan) for PCR, library preparation, and sequencing. PCR, library preparation, and Illumina MiSeq sequencing were prepared as described

ref:Individual and household attributes influence the dynamics of the personal skin microbiota and its association network

生信分析

16S 扩增子分辨率有限

网络分析